Anti-UBR4 Antibody (CAB12193)
- SKU:
- CAB12193
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-UBR4 Antibody |
| 抗体コード: | CAB12193 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 4884-5183 of human UBR4 (NP_065816.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:1000 |
| 反応性: | Human |
| ポジティブサンプル: | HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 4884-5183 of human UBR4 (NP_065816.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | NIVH YDCH LAAV RLAR GREE WESA ALQN ANTK CNGL LPVW GPHV PESA FATC LARH NTYL QECT GQRE PTYQ LNIH DIKL LFLR FAME QSFS ADTG GGGR ESNI HLIP YIIH TVLY VLNT TRAT SREE KNLQ GFLE QPKE KWVE SAFE VDGP YYFT VLAL HILP PEQW RATR VEIL RRLL VTSQ ARAV APGG ATRL TDKA VKDY SAYR SSLL FWAL VDLI YNMF KKVP TSNT EGGW SCSL AEYI RHND MPIY EAAD KALK TFQE EFMP VETF SEFL DVAG LLSE ITDP ESFL KDLL NSVP |
| 遺伝子ID: | 23352 |
| Uniprot: | Q5T4S7 |
| セルラーロケーション: | Cytoplasm, Membrane, Multi-pass membrane protein, Nucleus, cytoskeleton |
| 計算された分子量: | 23kDa/270kDa/571kDa/573kDa/575kDa |
| 観察された分子量: | 600kDa |
| 同義語: | UBR4, RBAF600, ZUBR1, p600 |
| バックグラウンド: | The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. |
| UniProt Protein Function: | UBR4: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. Belongs to the UBR4 family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Membrane protein, multi-pass; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 1p36.13 Cellular Component: centrosome; cytosol; membrane; nucleoplasm; plasma membrane Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: neutrophil degranulation; protein ubiquitination during ubiquitin-dependent protein catabolic process |
| NCBI Summary: | The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010] |
| UniProt Code: | Q5T4S7 |
| NCBI GenInfo Identifier: | 74744979 |
| NCBI Gene ID: | 23352 |
| NCBI Accession: | Q5T4S7.1 |
| UniProt Secondary Accession: | Q5T4S7,O60646, O75050, Q4QRK5, Q5T4S8, Q5T4S9, Q5TBN8 Q5TBP2, Q6DKH8, A8MPT2, A8MQ33, A8MQB1, |
| UniProt Related Accession: | Q5T4S7 |
| Molecular Weight: | 574kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase UBR4 |
| NCBI Synonym Full Names: | ubiquitin protein ligase E3 component n-recognin 4 |
| NCBI Official Symbol: | UBR4 |
| NCBI Official Synonym Symbols: | p600; ZUBR1; RBAF600 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase UBR4 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase UBR4 |
| UniProt Synonym Protein Names: | 600 kDa retinoblastoma protein-associated factor; N-recognin-4; RING-type E3 ubiquitin transferase UBR4; Retinoblastoma-associated factor of 600 kDa; RBAF600; p600; Zinc finger UBR1-type protein 1 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | UBR4 |
 | Western blot analysis of extracts of HeLa cells, using UBR4 antibody (CAB12193) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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