Anti-UBE2I Antibody (CAB13558)
- SKU:
- CAB13558
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-UBE2I Antibody |
| 抗体コード: | CAB13558 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human |
| ポジティブサンプル: | SW620 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSGI ALSR LAQE RKAW RKDH PFGF VAVP TKNP DGTM NLMN WECA IPGK KGTP WEGG LFKL RMLF KDDY PSSP PKCK FEPP LFHP NVYP SGTV CLSI LEED KDWR PAIT IKQI LLGI QELL NEPN IQDP AQAE AYTI YCQN RVEY EKRV RAQA KKFA PS |
| 遺伝子ID: | 7329 |
| Uniprot: | P63279 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 18kDa |
| 観察された分子量: | 18kDa |
| 同義語: | UBE2I, C358B7.1, P18, UBC9 |
| バックグラウンド: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5 |
| UniProt Protein Details: | Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine. Pathway: Protein modification; protein sumoylation. Subunit structure: Interacts with SIZ1. Ref.5 Ref.6 Subcellular location: Nucleus Ref.7. Miscellaneous: Present with 2600 molecules/cell in log phase SD medium. Ref.8 Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Code: | P63279 |
| NCBI GenInfo Identifier: | |
| NCBI Gene ID: | |
| NCBI Accession: | |
| Molecular Weight: | 17,911 Da |
| NCBI Full Name: | UBC9 |
| UniProt Protein Name: | SUMO-conjugating enzyme UBC9 |
| UniProt Synonym Protein Names: | Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase |
| UniProt Gene Name: | UBC9 |
| UniProt Entry Name: | UBC9_YEAST |
 | Western blot analysis of extracts of SW620 cells, using UBE2I Rabbit pAb (CAB13558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunofluorescence analysis of U2OS cells using UBE2I antibody (CAB13558). Blue: DAPI for nuclear staining. |