Anti-UBE2H Antibody (CAB7344)
- SKU:
- CAB7344
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-UBE2H Antibody |
| 抗体コード: | CAB7344 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2H (NP_003335.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SW480, BT-474, 293T, U-251MG, SKOV3, Mouse thymus, Mouse liver, Mouse lung, Rat lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2H (NP_003335.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSSP SPGK RRMD TDVV KLIE SKHE VTIL GGLN EFVV KFYG PQGT PYEG GVWK VRVD LPDK YPFK SPSI GFMN KIFH PNID EASG TVCL DVIN QTWT ALYD LTNI FESF LPQL LAYP NPID PLNG DAAA MYLH RPEE YKQK IKEY IQKY ATEE ALKE QEEG TGDS SSES SMSD FSED EAQD MEL |
| 遺伝子ID: | 7328 |
| Uniprot: | P62256 |
| セルラーロケーション: | |
| 計算された分子量: | 17kDa/20kDa |
| 観察された分子量: | 21-42kDa |
| 同義語: | UBE2H, E2-20K, GID3, UBC8, UBCH, UBCH2 |
| バックグラウンド: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. |
| UniProt Protein Function: | UBE2H: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A. Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; EC 6.3.2.19; Mitochondrial; Ubiquitin ligase; Ligase Chromosomal Location of Human Ortholog: 7q32 Cellular Component: cytoplasm Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process |
| NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011] |
| UniProt Code: | P62256 |
| NCBI GenInfo Identifier: | 51338683 |
| NCBI Gene ID: | 7328 |
| NCBI Accession: | P62256.1 |
| UniProt Secondary Accession: | P62256,P37286, Q7Z6F4, A4D1L6, C9JY93, |
| UniProt Related Accession: | P62256 |
| Molecular Weight: | 183 |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 H |
| NCBI Synonym Full Names: | ubiquitin-conjugating enzyme E2H |
| NCBI Official Symbol: | UBE2H |
| NCBI Official Synonym Symbols: | GID3; UBC8; UBCH; UBCH2; E2-20K |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 H; ubiquitin-protein ligase H; ubiquitin carrier protein H; GID complex subunit 3, UBC8 homolog; ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8) |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 H |
| UniProt Synonym Protein Names: | UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2H |
| UniProt Entry Name: | UBE2H_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBE2H antibody (CAB7344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A549 cells using UBE2H antibody (CAB7344). Blue: DAPI for nuclear staining. |
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