Anti-UBE2C Antibody (CAB18075)[KO Validated]

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SKU:
CAB18075
Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
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Description

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抗体名:Anti-UBE2C Antibody [KO Validated]
抗体コード:CAB18075
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC IF IP
反応性:Human
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).
申し込み:WB IHC IF IP
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50
反応性:Human
ポジティブサンプル:HeLa
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MASQ NRDP AATS VAAA RKGA EPSG GAAR GPVG KRLQ QELM TLMM SGDK GISA FPES DNLF KWVG TIHG AAGT VYED LRYK LSLE FPSG YPYN APTV KFLT PCYH PNVD TQGN ICLD ILKE KWSA LYDV RTIL LSIQ SLLG EPNI DSPL NTHA AELW KNPT AFKK YLQE TYSK QVTS QEP
遺伝子ID:11065
Uniprot:O00762
セルラーロケーション:
計算された分子量:15kDa/16kDa/17kDa/19kDa
観察された分子量:18kDa
Anti-UBE2C Antibody [KO Validated] (CAB18075)Western blot analysis of extracts from normal (control) and UBE2C knockout (KO) HeLa cells, using UBE2C antibody (CAB18075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
同義語:UBE2C, UBCH10, dJ447F3.2
バックグラウンド:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19.
UniProt Protein Function:UBE2C: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:

Protein type:Ligase; Cell cycle regulation; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: anaphase-promoting complex; cytoplasm; cytosol; nucleoplasm

Molecular Function:protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cyclin catabolic process; exit from mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of exit from mitosis; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of mitotic metaphase/anaphase transition; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process

NCBI Summary:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]
UniProt Code:O00762
NCBI GenInfo Identifier:3915178
NCBI Gene ID:11065
NCBI Accession:O00762.1
UniProt Secondary Accession:O00762,A6NP33, E1P5N7, G3XAB7,
UniProt Related Accession:O00762
Molecular Weight:17,373 Da
NCBI Full Name:Ubiquitin-conjugating enzyme E2 C
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 C
NCBI Official Symbol:UBE2C  
NCBI Official Synonym Symbols:UBCH10; dJ447F3.2  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 C
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 C
UniProt Synonym Protein Names:(E3-independent) E2 ubiquitin-conjugating enzyme C (EC:2.3.2.24
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2C  
UniProt Entry Name:UBE2C_HUMAN
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