Anti-TTL Antibody (CAB10027)
- SKU:
- CAB10027
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-TTL Antibody |
| 抗体コード: | CAB10027 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTL (NP_714923.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:4000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SH-SY5Y, HeLa, SKOV3, Mouse brain, Mouse lung, Mouse heart, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTL (NP_714923.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MYTF VVRD ENSS VYAE VSRL LLAT GHWK RLRR DNPR FNLM LGER NRLP FGRL GHEP GLVQ LVNY YRGA DKLC RKAS LVKL IKTS PELA ESCT WFPE SYVI YPTN LKTP VAPA QNGI QPPI SNSR TDER EFFL ASYN RKKE DGEG NVWI AKSS AGAK GEGI LISS EASE LLDF IDNQ GQVH VIQK YLEH PLLL EPGH RKFD IRSW VLVD HQYN IYLY REGV LRTA SEPY HVDN FQDK TCHL TNHC IQKE YSKN YGKY EEGN EMFF KEFN QY |
| 遺伝子ID: | 150465 |
| Uniprot: | Q8NG68 |
| セルラーロケーション: | |
| 計算された分子量: | 43kDa |
| 観察された分子量: | 43kDa |
| 同義語: | TTL |
| バックグラウンド: |
| UniProt Protein Function: | TTL: Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin. Belongs to the tubulin--tyrosine ligase family. |
| UniProt Protein Details: | Protein type:EC 6.3.2.25; Ligase; Cell development/differentiation Chromosomal Location of Human Ortholog: 2q13 |
| NCBI Summary: | TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q8NG68 |
| NCBI GenInfo Identifier: | 47117358 |
| NCBI Gene ID: | 150465 |
| NCBI Accession: | Q8NG68.2 |
| UniProt Secondary Accession: | Q8NG68,Q585T3, Q7Z302, Q8N426, |
| UniProt Related Accession: | Q8NG68 |
| Molecular Weight: | 43,212 Da |
| NCBI Full Name: | Tubulin--tyrosine ligase |
| NCBI Synonym Full Names: | tubulin tyrosine ligase |
| NCBI Official Symbol: | TTL |
| NCBI Protein Information: | tubulin--tyrosine ligase |
| UniProt Protein Name: | Tubulin--tyrosine ligase |
| Protein Family: | TITAN-like protein |
| UniProt Gene Name: | TTL |
| UniProt Entry Name: | TTL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TTL antibody (CAB10027) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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