Anti-TRIM63 Antibody (CAB3101)
- SKU:
- CAB3101
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-TRIM63 Antibody |
| 抗体コード: | CAB3101 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide of human TRIM63 |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse heart, Rat heart |
| 免疫原: | A synthetic peptide of human TRIM63 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 84676 |
| Uniprot: | Q969Q1 |
| セルラーロケーション: | Cytoplasm, M line, Nucleus, Z line, myofibril, sarcomere |
| 計算された分子量: | 36kDa/40kDa |
| 観察された分子量: | 40kDa |
| 同義語: | TRIM63, IRF, MURF1, MURF2, RNF28, SMRZ |
| バックグラウンド: | This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. |
| UniProt Protein Function: | MURF1: E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric- associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1p34-p33 Cellular Component: microtubule; cytoplasm; M band; Z disc; nucleus Molecular Function:signal transducer activity; protein binding; zinc ion binding; ubiquitin-protein ligase activity; titin binding; ligase activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; skeletal muscle atrophy; muscle contraction; regulation of gene expression; protein ubiquitination; response to electrical stimulus involved in regulation of muscle adaptation; signal transduction |
| NCBI Summary: | This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q969Q1 |
| NCBI GenInfo Identifier: | 21362898 |
| NCBI Gene ID: | 84676 |
| NCBI Accession: | Q969Q1.1 |
| UniProt Related Accession: | Q969Q1 |
| Molecular Weight: | 40.2kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase TRIM63 |
| NCBI Synonym Full Names: | tripartite motif containing 63 |
| NCBI Official Symbol: | TRIM63 |
| NCBI Official Synonym Symbols: | IRF; SMRZ; MURF1; MURF2; RNF28 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase TRIM63 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase TRIM63 |
| UniProt Synonym Protein Names: | Iris RING finger protein; Muscle-specific RING finger protein 1; MuRF-1; MuRF1; RING finger protein 28; Striated muscle RING zinc finger protein; Tripartite motif-containing protein 63 |
| UniProt Gene Name: | TRIM63 |
| UniProt Entry Name: | TRI63_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TRIM63 antibody (CAB3101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of A549 cells using TRIM63 antibody (CAB3101). Blue: DAPI for nuclear staining. |