Anti-TRIM25 Antibody (CAB19887)[KO Validated]
![Anti-TRIM25 Antibody (CAB19887)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/28673/28521/anti-trim25-antibody-cab19887ko-validated__94199__61200.1645807093.jpg?c=1 "Anti-TRIM25 Antibody (CAB19887)[KO Validated]")
- SKU:
- CAB19887
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
![Anti-TRIM25 Antibody (CAB19887)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/28673/28521/anti-trim25-antibody-cab19887ko-validated__94199__61200.1645807093.jpg?c=1&imbypass=on "Anti-TRIM25 Antibody (CAB19887)[KO Validated]")
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Description
| 抗体名: | Anti-TRIM25 Antibody [KO Validated] |
| 抗体コード: | CAB19887 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IP |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human TRIM25. |
| 申し込み: | WB IHC IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | A-549 |
| 免疫原: | Recombinant protein of human TRIM25. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 7706 |
| Uniprot: | Q14258 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 70kDa |
| 観察された分子量: | 71KDa |
![Anti-TRIM25 Antibody [KO Validated] (CAB19887)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/z/341/A19887_1__58291.jpg) | Western blot analysis of extracts from normal (control) and TRIM25 knockout (KO) A-549 cells, using TRIM25 antibody (CAB19887) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10S. |
| 同義語: | TRIM25, EFP, RNF147, Z147, ZNF147 |
| バックグラウンド: | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. |
| UniProt Protein Function: | TRIM25: a ubiquitous protein that mediates estrogen action in various target organs. Contains one RING-type zinc finger and one SPRY domain. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Ubiquitin conjugating system; Transcription factor; Ubiquitin ligase; EC 6.3.2.n3; Ligase; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 17q23.2 Cellular Component: nucleoplasm; cytoplasm; cytosol Molecular Function:protein binding; acid-amino acid ligase activity; zinc ion binding; transcription factor activity Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; protein ubiquitination; regulation of virion penetration into host cell; activation of NF-kappaB transcription factor; response to vitamin D; response to estrogen stimulus; innate immune response; positive regulation of transcription factor activity; defense response to virus; negative regulation of interferon type I production |
| NCBI Summary: | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14258 |
| NCBI GenInfo Identifier: | 313104033 |
| NCBI Gene ID: | 7706 |
| NCBI Accession: | Q14258.2 |
| UniProt Related Accession: | Q14258 |
| Molecular Weight: | 630 |
| NCBI Full Name: | E3 ubiquitin/ISG15 ligase TRIM25 |
| NCBI Synonym Full Names: | tripartite motif containing 25 |
| NCBI Official Symbol: | TRIM25 |
| NCBI Official Synonym Symbols: | EFP; Z147; RNF147; ZNF147 |
| NCBI Protein Information: | E3 ubiquitin/ISG15 ligase TRIM25; RING finger protein 147; zinc finger protein-147; tripartite motif-containing 25; tripartite motif protein TRIM25; estrogen-responsive finger protein; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147 (estrogen-responsive finger protein) |
| UniProt Protein Name: | E3 ubiquitin/ISG15 ligase TRIM25 |
| UniProt Synonym Protein Names: | Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147 |
| Protein Family: | E3 ubiquitin/ISG15 ligase |
| UniProt Gene Name: | TRIM25 |
| UniProt Entry Name: | TRI25_HUMAN |