Anti-TRIB3 Antibody (CAB5424)
- SKU:
- CAB5424
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| 抗体名: | Anti-TRIB3 Antibody |
| 抗体コード: | CAB5424 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TRIB3 (NP_066981.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, SK-HEP1, K-562, SKOV3, BT-474, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TRIB3 (NP_066981.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MRAT PLAA PAGS LSRK KRLE LDDN LDTE RPVQ KRAR SGPQ PRLP PCLL PLSP PTAP DRAT AVAT ASRL GPYV LLEP EEGG RAYQ ALHC PTGT EYTC KVYP VQEA LAVL EPYA RLPP HKHV ARPT EVLA GT |
| 遺伝子ID: | 57761 |
| Uniprot: | Q96RU7 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 39kDa |
| 観察された分子量: | 35kDa |
| 同義語: | TRIB3, C20orf97, NIPK, SINK, SKIP3, TRB3 |
| バックグラウンド: | The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. |
| UniProt Protein Function: | TRB3: a Ca(2)/calmodulin-dependent protein kinase of the Trbl family. Induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. May negatively regulate the cell survival kinase AKT1. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; Trbl family Chromosomal Location of Human Ortholog: 20p13-p12.2 Cellular Component: nucleoplasm; plasma membrane; cytosol; nucleus Molecular Function:protein binding; protein kinase inhibitor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity; kinase activity; transcription corepressor activity; protein kinase binding; ATP binding Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of protein binding; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; protein amino acid phosphorylation; cellular response to insulin stimulus; regulation of MAP kinase activity; positive regulation of ubiquitin-protein ligase activity; insulin receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; negative regulation of transcription, DNA-dependent; negative regulation of fatty acid biosynthetic process |
| NCBI Summary: | The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q96RU7 |
| NCBI GenInfo Identifier: | 28201830 |
| NCBI Gene ID: | 57761 |
| NCBI Accession: | Q96RU7.2 |
| UniProt Related Accession: | Q96RU7 |
| Molecular Weight: | 35kDa |
| NCBI Full Name: | Tribbles homolog 3 |
| NCBI Synonym Full Names: | tribbles pseudokinase 3 |
| NCBI Official Symbol: | TRIB3 |
| NCBI Official Synonym Symbols: | NIPK; SINK; TRB3; SKIP3; C20orf97 |
| NCBI Protein Information: | tribbles homolog 3 |
| UniProt Protein Name: | Tribbles homolog 3 |
| UniProt Synonym Protein Names: | Neuronal cell death-inducible putative kinase; SINK; p65-interacting inhibitor of NF-kappa-B |
| Protein Family: | Tribbles |
| UniProt Gene Name: | TRIB3 |
| UniProt Entry Name: | TRIB3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TRIB3 antibody (CAB5424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |