Anti-TRH Antibody (CAB14472)
- SKU:
- CAB14472
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-TRH Antibody |
| 抗体コード: | CAB14472 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRH (NP_009048.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, BxPC-3, A-549, DU145, Mouse brain, Rat testis |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRH (NP_009048.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EEEE EGGA VGPH KRQH PGRR EDEA SWSV DVTQ HKRQ HPGR RSPW LAYA VPKR QHPG RRLA DPKA QRSW EEEE EEEE REED LMPE KRQH PGKR ALGG PCGP Q |
| 遺伝子ID: | 7200 |
| Uniprot: | P20396 |
| セルラーロケーション: | Secreted |
| 計算された分子量: | 27kDa |
| 観察された分子量: | 27kDa |
| 同義語: | TRH, Pro-TRH, TRF |
| バックグラウンド: | This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. |
| UniProt Protein Function: | TRH: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes. Belongs to the TRH family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3q13.3-q21 Cellular Component: extracellular region; nucleus; plasma membrane; secretory granule Biological Process: cell-cell signaling; eating behavior; histamine metabolic process; negative regulation of glutamate secretion; positive regulation of gamma-aminobutyric acid secretion; positive regulation of insulin secretion; signal transduction Disease: Thyrotropin-releasing Hormone Deficiency |
| NCBI Summary: | This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013] |
| UniProt Code: | P20396 |
| NCBI GenInfo Identifier: | 135841 |
| NCBI Gene ID: | 7200 |
| NCBI Accession: | P20396.1 |
| UniProt Secondary Accession: | P20396,Q2TB83, B2R8R1, |
| UniProt Related Accession: | P20396 |
| Molecular Weight: | 27,404 Da |
| NCBI Full Name: | Pro-thyrotropin-releasing hormone |
| NCBI Synonym Full Names: | thyrotropin releasing hormone |
| NCBI Official Symbol: | TRH |
| NCBI Official Synonym Symbols: | TRF; Pro-TRH |
| NCBI Protein Information: | thyrotropin releasing hormone |
| UniProt Protein Name: | Pro-thyrotropin-releasing hormone |
| UniProt Synonym Protein Names: | Prothyroliberin |
| Protein Family: | Thyrotropin-releasing hormone |
| UniProt Gene Name: | TRH |
| UniProt Entry Name: | TRH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TRH antibody (CAB14472) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using TRH Rabbit pAb (CAB14472) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using TRH Rabbit pAb (CAB14472) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of H9C2 cells using TRH Rabbit pAb (CAB14472) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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