Anti-TPM2 Antibody (CAB3096)
- SKU:
- CAB3096
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-TPM2 Antibody |
| 抗体コード: | CAB3096 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide of human TPM2 |
| 申し込み: | WB IHC IF IP |
| 推奨希釈: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SW620, HeLa, MCF7, U-87MG, Mouse skeletal muscle, Mouse lung |
| 免疫原: | A synthetic peptide of human TPM2 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 7169 |
| Uniprot: | P07951 |
| セルラーロケーション: | Cytoplasm, cytoskeleton |
| 計算された分子量: | 28kDa/32kDa |
| 観察された分子量: | 36kDa |
| 同義語: | TPM2, AMCD1, DA1, DA2B, HEL-S-273, NEM4, TMSB |
| バックグラウンド: | This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | TPM2: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization. Defects in TPM2 are the cause of nemaline myopathy type 4 (NEM4). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life. Defects in TPM2 are the cause of distal arthrogryposis type 1A (DA1A). A form of distal arthrogryposis, a disease characterized by congenital joint flexures or contractures that mainly involve the distal parts of the limbs, and affect two or more different body areas in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Belongs to the tropomyosin family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 9p13 Cellular Component: cytosol; muscle thin filament tropomyosin Molecular Function:actin binding; structural constituent of muscle Biological Process: muscle contraction; muscle filament sliding; regulation of ATPase activity Disease: Arthrogryposis, Distal, Type 1a; Arthrogryposis, Distal, Type 2b; Myopathy, Congenital, With Fiber-type Disproportion; Nemaline Myopathy 4 |
| NCBI Summary: | This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009] |
| UniProt Code: | P07951 |
| NCBI GenInfo Identifier: | 136090 |
| NCBI Gene ID: | 7169 |
| NCBI Accession: | P07951.1 |
| UniProt Secondary Accession: | P07951,P06468, Q13894, Q53FM4, Q5TCU4, Q5TCU7, Q9UH67 A6NM85, |
| UniProt Related Accession: | P07951 |
| Molecular Weight: | 28,684 Da |
| NCBI Full Name: | Tropomyosin beta chain |
| NCBI Synonym Full Names: | tropomyosin 2 (beta) |
| NCBI Official Symbol: | TPM2 |
| NCBI Official Synonym Symbols: | DA1; DA2B; NEM4; TMSB; AMCD1; HEL-S-273 |
| NCBI Protein Information: | tropomyosin beta chain |
| UniProt Protein Name: | Tropomyosin beta chain |
| UniProt Synonym Protein Names: | Beta-tropomyosin; Tropomyosin-2 |
| Protein Family: | Tropomyosin |
| UniProt Gene Name: | TPM2 |
| UniProt Entry Name: | TPM2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TPM2 antibody (CAB3096) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat heart using TPM2 antibody (CAB3096) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using TPM2 antibody (CAB3096) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using TPM2 antibody (CAB3096) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 300ug extracts of Mouse skeletal muscle cells using 3ug TPM2 antibody (CAB3096). Western blot was performed from the immunoprecipitate using TPM2 antibody (CAB3096) at a dilition of 1:1000. |
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