Anti-TPM1 Antibody (CAB1157)
- SKU:
- CAB1157
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Zebrafish
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-TPM1 Antibody |
| 抗体コード: | CAB1157 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat, Zebrafish |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPM1 (NP_001018008.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat, Zebrafish |
| ポジティブサンプル: | A-549, BT-474, THP-1, DU145, SW480, MCF7, Mouse heart, Mouse skeletal muscle, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPM1 (NP_001018008.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAGS SSLE AVRR KIRS LQEQ ADAA EERA GTLQ RELD HERK LRET AEAD VASL NRRI QLVE EELD RAQE RLAT ALQK LEEA EKAA DESE RGMK VIES RAQK DEEK MEIQ EIQL KEAK HIAE DADR KYEE VARK LVII ESDL ERAE ERAE LSEG KCAE LEEE LKTV TNNL KSLE AQAE KYSQ KEDR YEEE IKVL SDKL KEAE TRAE FAER SVTK LEKS IDDL EDQL YQQL EQNR RLTN ELKL ALNE D |
| 遺伝子ID: | 7168 |
| Uniprot: | P09493 |
| セルラーロケーション: | Cytoplasm, cytoskeleton |
| 計算された分子量: | 26kDa/28kDa/32kDa |
| 観察された分子量: | 36kDa |
| 同義語: | TPM1, C15orf13, CMD1Y, CMH3, HEL-S-265, HTM-alpha, LVNC9, TMSA |
| バックグラウンド: | This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. |
| UniProt Protein Function: | TPM1: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Actin-binding; Motor Chromosomal Location of Human Ortholog: 15q22.1 Cellular Component: filamentous actin; sarcomere; cytoskeleton; stress fiber; cytosol; muscle thin filament tropomyosin Molecular Function:structural constituent of cytoskeleton; structural constituent of muscle; cytoskeletal protein binding; actin binding Biological Process: positive regulation of cell adhesion; wound healing; regulation of muscle contraction; in utero embryonic development; cytoskeleton organization and biogenesis; sarcomere organization; regulation of heart contraction; muscle filament sliding; muscle contraction; positive regulation of ATPase activity; positive regulation of stress fiber formation; ruffle organization and biogenesis; ventricular cardiac muscle morphogenesis; cell motility; negative regulation of cell migration; positive regulation of heart rate by epinephrine; cardiac muscle contraction Disease: Cardiomyopathy, Dilated, 1y; Cardiomyopathy, Familial Hypertrophic, 3 |
| NCBI Summary: | This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09493 |
| NCBI GenInfo Identifier: | 136092 |
| NCBI Gene ID: | 7168 |
| NCBI Accession: | P09493.2 |
| UniProt Secondary Accession: | P09493,P09494, P10469, Q6DV89, Q6DV90, Q7Z6L8, Q86W64 Q96IK2, B7Z5T7, D9YZV2, D9YZV3, D9YZV8, |
| UniProt Related Accession: | P09493 |
| Molecular Weight: | 284 |
| NCBI Full Name: | Tropomyosin alpha-1 chain |
| NCBI Synonym Full Names: | tropomyosin 1 (alpha) |
| NCBI Official Symbol: | TPM1 |
| NCBI Official Synonym Symbols: | CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HTM-alpha |
| NCBI Protein Information: | tropomyosin alpha-1 chain; alpha-tropomyosin; sarcomeric tropomyosin kappa; cardiomyopathy, hypertrophic 3 |
| UniProt Protein Name: | Tropomyosin alpha-1 chain |
| UniProt Synonym Protein Names: | Alpha-tropomyosin; Tropomyosin-1 |
| Protein Family: | Tropomyosin |
| UniProt Gene Name: | TPM1 |
| UniProt Entry Name: | TPM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TPM1 antibody (CAB1157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human lung using TPM1 antibody (CAB1157) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human appendix using TPM1 antibody (CAB1157) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human uterus using TPM1 antibody (CAB1157) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using TPM1 antibody (CAB1157). Blue: DAPI for nuclear staining. |
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