Anti-TPBG Antibody (RACO0495)
- SKU:
- RACO0495
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| 商品名: | TPBG Antibody |
| Product SKU: | RACO0495 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human, Mouse |
| 免疫原: | A synthesized peptide derived from human 5T4 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. |
| シノニム: | Trophoblast glycoprotein (5T4 oncofetal antigen) (5T4 oncofetal trophoblast glycoprotein) (5T4 oncotrophoblast glycoprotein) (M6P1) (Wnt-activated inhibitory factor 1) (WAIF1), TPBG, 5T4 |
 | IHC image of RACO0495 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in U-87 whole cell lysate A549 whole cell lysate MCF-7 whole cell lysate PC-3 whole cell lysate U-251 whole cell lysate Mouse Brain whole cell lysate All lanes 5T4 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 47 kDa. Observed band size: 50, 80 kDa. |
| UniProt Protein Function: | Subcellular location: Membrane; Single-pass type I membrane protein |
| UniProt Protein Details: | Potential. Tissue specificity: Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma. Miscellaneous: Antigen 5T4 is overexpressed by a wide spectrum of cancers, including colorectal, ovarian and gastric, but with a limited normal tissue expression. Could be used for tumor immunotherapy. Sequence similarities: Contains 6 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.Contains 1 LRRNT domain. |
| NCBI Summary: | This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q13641 |
| NCBI GenInfo Identifier: | 262205665 |
| NCBI Gene ID: | 7162 |
| NCBI Accession: | NP_001159864.1 |
| UniProt Related Accession: | Q13641 |
| Molecular Weight: | 46,032 Da |
| NCBI Full Name: | trophoblast glycoprotein |
| NCBI Synonym Full Names: | trophoblast glycoprotein |
| NCBI Official Symbol: | TPBG |
| NCBI Official Synonym Symbols: | 5T4; M6P1; 5T4AG |
| NCBI Protein Information: | trophoblast glycoprotein; 5T4 oncofetal antigen; 5T4 oncotrophoblast glycoprotein; 5T4 oncofetal trophoblast glycoprotein |
| UniProt Protein Name: | Trophoblast glycoprotein |
| UniProt Synonym Protein Names: | 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; M6P1 |
| Protein Family: | Trophoblast glycoprotein |
| UniProt Gene Name: | TPBG |
| UniProt Entry Name: | TPBG_HUMAN |