Anti-TOPBP1 Antibody (CAB12574)
- SKU:
- CAB12574
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-TOPBP1 Antibody |
| 抗体コード: | CAB12574 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOPBP1 (NP_008958.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Jurkat, K-562 |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOPBP1 (NP_008958.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSRN DKEP FFVK FLKS SDNS KCFF KALE SIKE FQSE EYLQ IITE EEAL KIKE NDRS LYIC DPFS GVVF DHLK KLGC RIVG PQVV IFCM HHQR CVPR AEHP |
| 遺伝子ID: | 11073 |
| Uniprot: | Q92547 |
| セルラーロケーション: | Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle pole |
| 計算された分子量: | 170kDa |
| 観察された分子量: | 171kDa |
| 同義語: | TOPBP1, TOP2BP1 |
| バックグラウンド: | This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. |
| UniProt Protein Function: | TOPBP1: a DNA-binding protein and scaffolding protein that is required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. Contains 7 BRCT domains, which are characteristic of proteins involved in cell cycle checkpoint control in response to DNA damage. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase. Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon. |
| UniProt Protein Details: | Protein type:DNA replication Chromosomal Location of Human Ortholog: 3q22.1 Cellular Component: male germ cell nucleus; Golgi apparatus; spindle pole; centrosome; PML body; condensed nuclear chromosome; cytoplasm; chromosome; nucleus; actin cytoskeleton Molecular Function:protein C-terminus binding; identical protein binding; protein binding; DNA binding Biological Process: response to ionizing radiation; DNA repair; response to DNA damage stimulus; DNA metabolic process |
| NCBI Summary: | This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92547 |
| NCBI GenInfo Identifier: | 296453012 |
| NCBI Gene ID: | 11073 |
| NCBI Accession: | Q92547.3 |
| UniProt Secondary Accession: | Q92547,Q7LGC1, Q9UEB9, B7Z7W8, |
| UniProt Related Accession: | Q92547 |
| Molecular Weight: | 1522 |
| NCBI Full Name: | DNA topoisomerase 2-binding protein 1 |
| NCBI Synonym Full Names: | topoisomerase (DNA) II binding protein 1 |
| NCBI Official Symbol: | TOPBP1 |
| NCBI Official Synonym Symbols: | TOP2BP1 |
| NCBI Protein Information: | DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-binding protein 1; DNA topoisomerase II-beta-binding protein 1 |
| UniProt Protein Name: | DNA topoisomerase 2-binding protein 1 |
| UniProt Synonym Protein Names: | DNA topoisomerase II-beta-binding protein 1; TopBP1; DNA topoisomerase II-binding protein 1 |
| Protein Family: | DNA topoisomerase 2-binding protein |
| UniProt Gene Name: | TOPBP1 |
| UniProt Entry Name: | TOPB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TOPBP1 antibody (CAB12574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using TOPBP1 antibody (CAB12574) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat lung using TOPBP1 antibody (CAB12574) at dilution of 1:100 (40x lens). |
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