Anti-TOP1 Antibody (CAB12524)
- SKU:
- CAB12524
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-TOP1 Antibody |
| 抗体コード: | CAB12524 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IP |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP1 (NP_003277.1). |
| 申し込み: | WB IHC IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, SKOV3, SW480, Jurkat, HeLa, Mouse spleen, Mouse ovary |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP1 (NP_003277.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSGD HLHN DSQI EADF RLND SHKH KDKH KDRE HRHK EHKK EKDR EKSK HSNS EHKD SEKK HKEK EKTK HKDG SSEK HKDK HKDR DKEK RKEE KVRA SGDA |
| 遺伝子ID: | 7150 |
| Uniprot: | P11387 |
| セルラーロケーション: | Nucleus, nucleolus, nucleoplasm |
| 計算された分子量: | 90kDa |
| 観察された分子量: | 120kDa |
| 同義語: | TOP1, TOPI |
| バックグラウンド: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. |
| UniProt Protein Function: | TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family. |
| UniProt Protein Details: | Protein type:EC 5.99.1.2; Isomerase; Nucleolus; Oncoprotein Chromosomal Location of Human Ortholog: 20q12 Cellular Component: nuclear chromosome; nucleolus; nucleoplasm; nucleus Molecular Function:chromatin binding; DNA binding; DNA topoisomerase type I activity; protein binding Biological Process: chromatin remodeling; chromosome segregation; circadian regulation of gene expression; circadian rhythm; DNA replication; DNA topological change; protein sumoylation; response to drug |
| NCBI Summary: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P11387 |
| NCBI GenInfo Identifier: | 12644118 |
| NCBI Gene ID: | 7150 |
| NCBI Accession: | P11387.2 |
| UniProt Secondary Accession: | P11387,O43256, Q12855, Q12856, Q15610, Q5TFY3, Q9UJN0 A8KA78, E1P5W3, |
| UniProt Related Accession: | P11387 |
| Molecular Weight: | 90,726 Da |
| NCBI Full Name: | DNA topoisomerase 1 |
| NCBI Synonym Full Names: | topoisomerase (DNA) I |
| NCBI Official Symbol: | TOP1 |
| NCBI Official Synonym Symbols: | TOPI |
| NCBI Protein Information: | DNA topoisomerase 1 |
| UniProt Protein Name: | DNA topoisomerase 1 |
| UniProt Synonym Protein Names: | DNA topoisomerase I |
| Protein Family: | Crescerin |
| UniProt Gene Name: | TOP1 |
 | Western blot analysis of extracts of various cell lines, using TOP1 antibody (CAB12524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse liver using TOP1 antibody (CAB12524) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug TOP1 antibody (CAB12524). Western blot was performed from the immunoprecipitate using TOP1 antibody (CAB12524) at a dilition of 1:1000. |
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