Anti-TONSL Antibody (CAB13035)
- SKU:
- CAB13035
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-TONSL Antibody |
| 抗体コード: | CAB13035 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-390 of human TONSL (NP_038460.4). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, Rat spleen, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-390 of human TONSL (NP_038460.4). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MRTR LYLN LGLT FESL QQTA LCND YFRK SIFL AEQN HLYE DLFR ARYN LGTI HWRA GQHS QAMR CLEG AREC AHTM RKRF MESE CCVV IAQV LQDL GDFL AAKR ALKK AYRL GSQK PVQR AAIC QNLQ HVLA VVRL QQQL EEAE GRDP QGAM VICE QLGD LFSK AGDF PRAA EAYQ KQLR FAEL LDRP GAER AIIH VSLA TTLG DMKD HHGA VRHY EEEL RLRS GNVL EEA |
| 遺伝子ID: | 4796 |
| Uniprot: | Q96HA7 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 150kDa |
| 観察された分子量: | 151kDa |
| 同義語: | TONSL, IKBR, NFKBIL2 |
| バックグラウンド: | The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus. |
| UniProt Protein Function: | NFKBIL2: Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork- associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold. Belongs to the Tonsoku family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: DNA replication factor A complex; MCM complex; nuclear replication fork; nucleoplasm Molecular Function:histone binding; protein binding; transcription corepressor activity Biological Process: cytoplasmic sequestering of transcription factor; double-strand break repair via homologous recombination; replication fork processing |
| NCBI Summary: | The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96HA7 |
| NCBI GenInfo Identifier: | 182662416 |
| NCBI Gene ID: | 4796 |
| NCBI Accession: | Q96HA7.2 |
| UniProt Secondary Accession: | Q96HA7,Q13006, Q9UGJ2, B5MDP0, C9JKB1, C9JNV8, |
| UniProt Related Accession: | Q96HA7 |
| Molecular Weight: | 150,894 Da |
| NCBI Full Name: | Tonsoku-like protein |
| NCBI Synonym Full Names: | tonsoku-like, DNA repair protein |
| NCBI Official Symbol: | TONSL |
| NCBI Official Synonym Symbols: | IKBR; NFKBIL2 |
| NCBI Protein Information: | tonsoku-like protein |
| UniProt Protein Name: | Tonsoku-like protein |
| UniProt Synonym Protein Names: | Inhibitor of kappa B-related protein; I-kappa-B-related protein; IkappaBR; NF-kappa-B inhibitor-like protein 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2 |
| UniProt Gene Name: | TONSL |
| UniProt Entry Name: | TONSL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TONSL antibody (CAB13035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of mouse brain, using TONSL antibody (CAB13035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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