Anti-TNNI1 Antibody (CAB4161)
- SKU:
- CAB4161
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-TNNI1 Antibody |
| 抗体コード: | CAB4161 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human TNNI1 (NP_003272.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse skeletal muscle, Rat skeletal muscle |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human TNNI1 (NP_003272.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPEV ERKP KITA SRKL LLKS LMLA KAKE CWEQ EHEE REAE KVRY LAER IPTL QTRG LSLS ALQD LCRE LHAK VEVV DEER YDIE AKCL HNTR EIKD LKLK |
| 遺伝子ID: | 7135 |
| Uniprot: | P19237 |
| セルラーロケーション: | |
| 計算された分子量: | 21kDa |
| 観察された分子量: | 22kDa |
| 同義語: | TNNI1, SSTNI, TNN1, troponin I1 |
| バックグラウンド: | Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. |
| UniProt Protein Function: | TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 1q31.3 Cellular Component: troponin complex; cytosol Molecular Function:metal ion binding; actin binding Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding |
| NCBI Summary: | Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P19237 |
| NCBI GenInfo Identifier: | 1351298 |
| NCBI Gene ID: | 7135 |
| NCBI Accession: | P19237.3 |
| UniProt Secondary Accession: | P19237,Q659A5, Q6FGS7, Q6FGW1, Q6ICU2, Q86T57, Q96DT9 A6NEH3, A8MSJ0, |
| UniProt Related Accession: | P19237 |
| Molecular Weight: | 187 |
| NCBI Full Name: | Troponin I, slow skeletal muscle |
| NCBI Synonym Full Names: | troponin I type 1 (skeletal, slow) |
| NCBI Official Symbol: | TNNI1 |
| NCBI Official Synonym Symbols: | TNN1; SSTNI |
| NCBI Protein Information: | troponin I, slow skeletal muscle; troponin I, slow-twitch isoform |
| UniProt Protein Name: | Troponin I, slow skeletal muscle |
| UniProt Synonym Protein Names: | Troponin I, slow-twitch isoform |
| Protein Family: | Troponin |
| UniProt Gene Name: | TNNI1 |
| UniProt Entry Name: | TNNI1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TNNI1 antibody (CAB4161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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