Anti-TMPO 抗体 (CAB2534)

抗体名:	Anti-TMPO Antibody [KO Validated]
抗体コード:	CAB2534
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	MCF7, 293T, HepG2, mouse liver, rat liver

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MPEF LEDP SVLT KDKL KSEL VANN VTLP AGEQ RKDV YVQL YLQH LTAR NRPP LPAG TNSK GPPD FSSD EERE PTPV LGSG AAAA GRSR AAVG RKAT KKTD KPRQ EDKD DLDV TELT NEDL LDQL VKYG VNPG PIVG TTRK LYEK KLLK LREQ GTES RSST PLPT ISSS AENT RQNG SNDS DRYS DNEE DSKI ELKL EKRE PLKG RAKT PVTL KQRR VEHN QSYS QAGI TETE WTSG SSKG GPLQ ALTR ESTR GSRR TPRK RVET SEHF RIDG PVIS ESTP IAET IMAS SNES LVVN RVTG NFKH ASPI LPIT EFSD IPRR APKK PLTR AEVG EKTE ERRV ERDI LKEM FPYE ASTP TGIS ASCR RPIK GAAG RPLE LSDF RMEE SFSS KYVP KYVP LADV KSEK TKKG RS
遺伝子ID:	7112
Uniprot:	P42166/P42167
セルラーロケーション:	Chromosome, Nucleus
計算された分子量:	38kDa/50kDa/75kDa
観察された分子量:	51kDa/80kDa

同義語:

TMPO, CMD1T, LAP2, LEMD4, PRO0868, TP

バックグラウンド:

The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

UniProt Protein Function:	TMPO iso6: a single-pass type II membrane protein. Tightly associated with the nuclear lamina. Binds DNA and involved in DNA replication and chromatin remodeling, May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with HAP95. Associated with dilated cardiomyopathy; upregulated in medulloblastoma. Used as a pharmaceutical to treat primary and secondary immune deficiencies, autoimmunity, infections and cancer. Available under the names Timunox (Cilag), Sintomodulina (Italofarmaco) and Mepentil (Recordati). Three alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:DNA-binding; Membrane protein, integral Chromosomal Location of Human Ortholog: 12q22 Cellular Component: chromatin; nuclear envelope; nucleus Molecular Function:lamin binding Disease: Cardiomyopathy, Dilated, 1t
NCBI Summary:	The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, May 2010]
UniProt Code:	P42166
NCBI GenInfo Identifier:	1174689
NCBI Gene ID:	7112
NCBI Accession:	P42166.2
UniProt Secondary Accession:	P42166,P08918, P08919, Q14860, Q16295,
UniProt Related Accession:	P42166,P42167
Molecular Weight:	50,670 Da
NCBI Full Name:	Lamina-associated polypeptide 2, isoform alpha
NCBI Synonym Full Names:	thymopoietin
NCBI Official Symbol:	TMPO
NCBI Official Synonym Symbols:	TP; LAP2; CMD1T; LEMD4; PRO0868
NCBI Protein Information:	thymopoietin
UniProt Protein Name:	Lamina-associated polypeptide 2, isoform alpha
UniProt Synonym Protein Names:	Thymopoietin isoform alpha; TP alpha
Protein Family:	Lamina-associated polypeptide
UniProt Gene Name:	TMPO
UniProt Entry Name:	LAP2A_HUMAN

	Western blot analysis of extracts of various cell lines, using TMPO antibody (CAB2534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (CAB2534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (CAB2534) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (CAB2534) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (CAB2534) at dilution of 1:100 (40x lens).

Antibodies

TMPO/TMPO Antibody (PACO07294)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)