Anti-TIMM8B 抗体 (CAB15814)

抗体名:	Anti-TIMM8B Antibody
抗体コード:	CAB15814
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-98 of human TIMM8B (NP_036591.2).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
反応性:	Human, Mouse, Rat
ポジティブサンプル:	293T, A-549, MCF7, Mouse brain, Mouse kidney, Rat liver

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-98 of human TIMM8B (NP_036591.2).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MAEL GEAD EAEL QRLV AAEQ QKAQ FTAQ VHHF MELC WDKC VEKP GNRL DSRT ENCL SSCV DRFI DTTL AITS RFAQ IVQK GGQ
遺伝子ID:	26521
Uniprot:	Q9Y5J9
セルラーロケーション:	Intermembrane side, Mitochondrion inner membrane, Peripheral membrane protein
計算された分子量:	9kDa
観察された分子量:	12kDa

同義語:

TIMM8B, DDP2, TIM8B

バックグラウンド:

This gene encodes a member of a well-conserved family of proteins with similarity to yeast Tim mitochondrial import proteins. This gene is encoded by a nuclear gene and is transported into the intermembrane space of the mitochondrion. When formed into complexes, these proteins guide membrane-spanning proteins across the mitochondrial intermembrane space before they are added into the mitochondrial inner membrane. This gene is adjacent to succinate dehydrogenase, subunit D (SDHD), in which mutations have been found in affected members of families with hereditary paraganglioma.

UniProt Protein Function:	Function: Probable mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space
UniProt Protein Details:	By similarity. Subunit structure: Heterohexamer; possibly composed of 3 copies of TIMM8B and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 By similarity. Subcellular location: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity. Tissue specificity: Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle. Ref.2 Domain: The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM8B from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity. Sequence similarities: Belongs to the small Tim family.
NCBI Summary:	This gene encodes a member of a well-conserved family of proteins with similarity to yeast Tim mitochondrial import proteins. This gene is encoded by a nuclear gene and is transported into the intermembrane space of the mitochondrion. When formed into complexes, these proteins guide membrane-spanning proteins across the mitochondrial intermembrane space before they are added into the mitochondrial inner membrane. This gene is adjacent to succinate dehydrogenase, subunit D (SDHD), in which mutations have been found in affected members of families with hereditary paraganglioma.[provided by RefSeq, Aug 2009]
UniProt Code:	Q9Y5J9
NCBI GenInfo Identifier:	6524627
NCBI Gene ID:	26521
NCBI Accession:	AAF15100.1
UniProt Secondary Accession:	Q9Y5J9,P62077, P62078,
UniProt Related Accession:	Q9Y5J9
Molecular Weight:	9,344 Da
NCBI Full Name:	TIMM8b
NCBI Synonym Full Names:	translocase of inner mitochondrial membrane 8 homolog B (yeast)
NCBI Official Symbol:	TIMM8B
NCBI Official Synonym Symbols:	DDP2; TIM8B
NCBI Protein Information:	mitochondrial import inner membrane translocase subunit Tim8 B; DDP-like protein; deafness dystonia protein 2
UniProt Protein Name:	Mitochondrial import inner membrane translocase subunit Tim8 B
UniProt Synonym Protein Names:	DDP-like protein; Deafness dystonia protein 2
UniProt Gene Name:	TIMM8B
UniProt Entry Name:	TIM8B_HUMAN

	Western blot analysis of extracts of various cell lines, using TIMM8B antibody (CAB15814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using TIMM8B antibody (CAB15814) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using TIMM8B antibody (CAB15814) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using TIMM8B antibody (CAB15814) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using TIMM8B Polyclonal Antibody (CAB15814) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)