Anti-TIMM50 Antibody (CAB9991)
- SKU:
- CAB9991
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-TIMM50 Antibody |
| 抗体コード: | CAB9991 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-456 of human TIMM50 (NP_001001563.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:4000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | B cells, Mouse heart, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-456 of human TIMM50 (NP_001001563.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GNNP VDEN GAKI PDEF DNDP ILVQ QLRR TYKY FKDY RQMI IEPT SPCL LPDP LQEP YYQP PYTL VLEL TGVL LHPE WSLA TGWR FKKR PGIE TLFQ QLAP LYEI VIFT SETG MTAF PLID SVDP HGFI SYRL FRDA TRYM DGHH VKDI SCLN RDPA RVVV VDCK KEAF RLQP YNGV ALRP WDGN SDDR VLLD LSAF LKTI ALNG VEDV RTVL EHYA LEDD PLAA FKQR QSRL EQEE QQRL AELS KSNK QNLF LGSL TSRL WPRS KQP |
| 遺伝子ID: | 92609 |
| Uniprot: | Q3ZCQ8 |
| セルラーロケーション: | Mitochondrion inner membrane, Nucleus speckle, Single-pass membrane protein |
| 計算された分子量: | 27kDa/39kDa/50kDa |
| 観察された分子量: | 39kDa |
| 同義語: | TIMM50, TIM50, TIM50L |
| バックグラウンド: |
| UniProt Protein Function: | TIMM50: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. Belongs to the TIM50 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Mitochondrial; Protein phosphatase, dual-specificity Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: mitochondrial inner membrane; mitochondrial inner membrane presequence translocase complex; mitochondrion; nuclear speck; nucleoplasm Molecular Function:interleukin-2 receptor binding; phosphoprotein phosphatase activity; protein binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; ribonucleoprotein binding Biological Process: mitochondrial membrane organization and biogenesis; protein amino acid dephosphorylation; protein import into mitochondrial matrix; release of cytochrome c from mitochondria |
| NCBI Summary: | This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis. [provided by RefSeq, Jul 2016] |
| UniProt Code: | Q3ZCQ8 |
| NCBI GenInfo Identifier: | 83305924 |
| NCBI Gene ID: | 92609 |
| NCBI Accession: | Q3ZCQ8.2 |
| UniProt Secondary Accession: | Q3ZCQ8,Q330K1, Q6QA00, Q96FJ5, Q96GY2, Q9H370, |
| UniProt Related Accession: | Q3ZCQ8 |
| Molecular Weight: | 27,579 Da |
| NCBI Full Name: | Mitochondrial import inner membrane translocase subunit TIM50 |
| NCBI Synonym Full Names: | translocase of inner mitochondrial membrane 50 |
| NCBI Official Symbol: | TIMM50 |
| NCBI Official Synonym Symbols: | TIM50; TIM50L |
| NCBI Protein Information: | mitochondrial import inner membrane translocase subunit TIM50 |
| UniProt Protein Name: | Mitochondrial import inner membrane translocase subunit TIM50 |
| Protein Family: | Mitochondrial import inner membrane translocase |
| UniProt Gene Name: | TIMM50 |
| UniProt Entry Name: | TIM50_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TIMM50 antibody (CAB9991) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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