Anti-TIA1 Antibody (CAB12523)
- SKU:
- CAB12523
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| 抗体名: | Anti-TIA1 Antibody |
| 抗体コード: | CAB12523 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TIA1 (NP_071505.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | BT-474, Raji, Mouse brain, Mouse spleen |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TIA1 (NP_071505.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | IGYP QPYG QWGQ WYGN AQQI GQYM PNGW QVPA YGMY GQAW NQQG FNQT QSSA PWMG PNYG VQPP QGQN GSML PNQP SGYR VAGY ETQ |
| 遺伝子ID: | 7072 |
| Uniprot: | P31483 |
| セルラーロケーション: | Cytoplasmic granule, Nucleus |
| 計算された分子量: | 24kDa/41kDa/42kDa |
| 観察された分子量: | 43-50kDa |
| 同義語: | TIA1, TIA-1, WDM |
| バックグラウンド: | The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. |
| UniProt Protein Function: | TIA1: Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Translation; RNA-binding Chromosomal Location of Human Ortholog: 2p13 Cellular Component: nucleoplasm; stress granule; cytoplasm Molecular Function:protein binding; nucleotide binding; AU-rich element binding; poly(A) binding Biological Process: regulation of nuclear mRNA splicing, via spliceosome; apoptosis; negative regulation of translation; negative regulation of cytokine biosynthetic process Disease: Welander Distal Myopathy |
| NCBI Summary: | The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms has been found for this gene. [provided by RefSeq, May 2017] |
| UniProt Code: | P31483 |
| NCBI GenInfo Identifier: | 206729905 |
| NCBI Gene ID: | 7072 |
| NCBI Accession: | P31483.3 |
| UniProt Related Accession: | P31483 |
| Molecular Weight: | 43kDa |
| NCBI Full Name: | Nucleolysin TIA-1 isoform p40 |
| NCBI Synonym Full Names: | TIA1 cytotoxic granule associated RNA binding protein |
| NCBI Official Symbol: | TIA1 |
| NCBI Official Synonym Symbols: | WDM; TIA-1 |
| NCBI Protein Information: | nucleolysin TIA-1 isoform p40; nucleolysin TIA-1 |
| UniProt Protein Name: | Nucleolysin TIA-1 isoform p40 |
| UniProt Synonym Protein Names: | RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1; p40-TIA-1 |
| Protein Family: | Nucleolysin |
| UniProt Gene Name: | TIA1 |
| UniProt Entry Name: | TIA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TIA1 antibody (CAB12523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using TIA1 antibody (CAB12523) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using TIA1 antibody (CAB12523) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using TIA1 antibody (CAB12523) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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