Anti-TERT Antibody (CAB2979)
- SKU:
- CAB2979
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-TERT Antibody |
| 抗体コード: | CAB2979 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1000 to the C-terminus of human TERT (NP_937983.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | 293T, HL-60, MCF7, Rat thymus |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1000 to the C-terminus of human TERT (NP_937983.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | NIYK ILLL QAYR FHAC VLQL PFHQ QVWK NPTF FLRV ISDT ASLC YSIL KAKN AGMS LGAK GAAG PLPS EAVQ WLCH QAFL LKLT RHRV TYVP LLGS LRTA QTQL SRKL PGTT LTAL EAAA NPAL PSDF KTIL D |
| 遺伝子ID: | 7015 |
| Uniprot: | O14746 |
| セルラーロケーション: | Chromosome, Cytoplasm, Nucleus, PML body, nucleolus, nucleoplasm, telomere |
| 計算された分子量: | 88kDa/90kDa/120kDa/126kDa |
| 観察された分子量: | 127kDa |
| 同義語: | TERT, CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT |
| バックグラウンド: | Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. |
| UniProt Protein Function: | TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined. |
| UniProt Protein Details: | Protein type:Transferase; Nucleolus; EC 2.7.7.49; DNA-binding Chromosomal Location of Human Ortholog: 5p15.33 Cellular Component: nucleoplasm; PML body; chromosome, telomeric region; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolus Molecular Function:telomerase activity; protein binding; protein homodimerization activity; RNA-directed RNA polymerase activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; nucleotidyltransferase activity; tRNA binding Biological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; positive regulation of Wnt receptor signaling pathway; RNA interference, production of siRNA; telomere maintenance Disease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1 |
| NCBI Summary: | Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O14746 |
| NCBI GenInfo Identifier: | 6226780 |
| NCBI Gene ID: | 7015 |
| NCBI Accession: | O14746.1 |
| UniProt Secondary Accession: | O14746,O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46, |
| UniProt Related Accession: | O14746 |
| Molecular Weight: | 88,965 Da |
| NCBI Full Name: | Telomerase reverse transcriptase |
| NCBI Synonym Full Names: | telomerase reverse transcriptase |
| NCBI Official Symbol: | TERT |
| NCBI Official Synonym Symbols: | TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1 |
| NCBI Protein Information: | telomerase reverse transcriptase; telomerase catalytic subunit; telomerase-associated protein 2 |
| UniProt Protein Name: | Telomerase reverse transcriptase |
| UniProt Synonym Protein Names: | HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2; TP2 |
| Protein Family: | Telomerase reverse transcriptase |
| UniProt Gene Name: | TERT |
| UniProt Entry Name: | TERT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TERT antibody (CAB2979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 20s. |
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