Anti-TEAD4 Antibody (CAB4151)
- SKU:
- CAB4151
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-TEAD4 Antibody |
| 抗体コード: | CAB4151 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-250 of human TEAD4 (NP_003204.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:200 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, K-562, SW620, HeLa, Mouse lung, Mouse skeletal muscle, Mouse liver, Rat lung, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-250 of human TEAD4 (NP_003204.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AREI QAKL KDQA AKDK ALQS MAAM SSAQ IISA TAFH SSMA LARG PGRP AVSG FWQG ALPG QAGT SHDV KPFS QQTY AVQP PLPL PGFE SPAG PAPS PSAP PAPP WQGR SVAS SKLW MLEF SAFL EQQQ DPDT YNKH LFVH I |
| 遺伝子ID: | 7004 |
| Uniprot: | Q15561 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 34kDa/44kDa/48kDa |
| 観察された分子量: | 48kDa |
| 同義語: | TEAD4, EFTR-2, RTEF1, TCF13L1, TEF-3, TEF3, TEFR-1, hRTEF-1B |
| バックグラウンド: | This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. |
| UniProt Protein Function: | TEAD4: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 12p13.3-p13.2 Cellular Component: nucleoplasm Molecular Function:protein binding; DNA binding; transcription factor activity Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; muscle development; gene expression; skeletal development |
| NCBI Summary: | This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15561 |
| NCBI GenInfo Identifier: | 215274211 |
| NCBI Gene ID: | 7004 |
| NCBI Accession: | Q15561.3 |
| UniProt Related Accession: | Q15561 |
| Molecular Weight: | Observed Molecular Weight: (Observed)48kD, 60kD.Predicted Molecular Weight: (Calculated)48kDa. |
| NCBI Full Name: | Transcriptional enhancer factor TEF-3 |
| NCBI Synonym Full Names: | TEA domain transcription factor 4 |
| NCBI Official Symbol: | TEAD4 |
| NCBI Official Synonym Symbols: | TEF3; RTEF1; TEF-3; EFTR-2; TEFR-1; TCF13L1; hRTEF-1B |
| NCBI Protein Information: | transcriptional enhancer factor TEF-3 |
| UniProt Protein Name: | Transcriptional enhancer factor TEF-3 |
| UniProt Synonym Protein Names: | TEA domain family member 4; TEAD-4; Transcription factor 13-like 1; Transcription factor RTEF-1 |
| Protein Family: | Transcriptional enhancer factor |
| UniProt Gene Name: | TEAD4 |
| UniProt Entry Name: | TEAD4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TEAD4 antibody (CAB4151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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