Anti-TCEB3 Antibody (CAB6994)
- SKU:
- CAB6994
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-TCEB3 Antibody |
| 抗体コード: | CAB6994 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 679-798 of human TCEB3 (NP_003189.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | BT-474, HeLa, Raji, Mouse testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 679-798 of human TCEB3 (NP_003189.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ANKP KGRQ AKMA FVNS VAKP PRDV RRRQ EKFG TGGA AVPE KIKI KPAP YPMG SSHA SASS ISFN PSPE EPAY DGPS TSSA HLAP VVSS TVSY DPRK PTVK KIAP MMAK TIKA FKNR FSRR |
| 遺伝子ID: | 6924 |
| Uniprot: | Q14241 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 89kDa |
| 観察された分子量: | 105kDa |
| 同義語: | ELOA, SIII, SIII p110, TCEB3, TCEB3A, SIIIp110, elongin-A |
| バックグラウンド: | This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. |
| UniProt Protein Function: | TCEB3: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). Heterotrimer of an A (A1, A2 or A3), B and C subunit. The C subunit mediates the binding of the elongin BC complex to subunit A. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 1p36.1 Cellular Component: nucleoplasm; extracellular space; cytoplasm; integral to membrane; nucleus Molecular Function:DNA binding Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression |
| NCBI Summary: | This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14241 |
| NCBI GenInfo Identifier: | 294862430 |
| NCBI Gene ID: | 6924 |
| NCBI Accession: | Q14241.2 |
| UniProt Related Accession: | Q14241 |
| Molecular Weight: | ~ 105kDa |
| NCBI Full Name: | Elongin-A |
| NCBI Synonym Full Names: | elongin A |
| NCBI Official Symbol: | ELOA |
| NCBI Official Synonym Symbols: | SIII; TCEB3; TCEB3A; SIII p110 |
| NCBI Protein Information: | elongin-A |
| UniProt Protein Name: | Transcription elongation factor B polypeptide 3 |
| UniProt Synonym Protein Names: | Elongin 110 kDa subunit; Elongin-A; EloA; RNA polymerase II transcription factor SIII subunit A1; SIII p110 |
| Protein Family: | Protein |
| UniProt Gene Name: | TCEB3 |
| UniProt Entry Name: | ELOA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TCEB3 antibody (CAB6994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human kidney cancer using TCEB3 antibody (CAB6994) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using TCEB3 antibody (CAB6994). |
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