Anti-TAPBP 抗体 (CAB13357)

抗体名:	Anti-TAPBP Antibody
抗体コード:	CAB13357
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-260 of human TAPBP (NP_757345.2).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	THP-1, Raji, M21, Mouse liver

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-260 of human TAPBP (NP_757345.2).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	VEDA SGKG LAKR PGAL LLRQ GPGE PPPR PDLD PELY LSVH DPAG ALQA AFRR YPRG APAP HCEM SRFV PLPA SAKW ASGL TPAQ NCPR ALDG AWLM VSIS SPVL SLSS LLRP QPEP QQEP VLIT MATV VLTV LTHT PAPR VRLG QDAL LDLS FAYM PPTS EAAS SLAP GPPP FGLE WRRQ HLGK GHLL LAAT PGLN GQMP AAQE GAVA FAAW DDDE PWGP WTGN GTFW LPT
遺伝子ID:	6892
Uniprot:	O15533
セルラーロケーション:	Endoplasmic reticulum membrane, Single-pass type I membrane protein
計算された分子量:	38kDa/43kDa/47kDa/53kDa
観察された分子量:	48kDa

同義語:

TAPBP, NGS17, TAPA, TPN, TPSN, tapasin

バックグラウンド:

This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.

UniProt Protein Function:	TAPBP: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading). 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: Golgi membrane; endoplasmic reticulum membrane; TAP complex; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; peptide antigen binding; TAP1 binding; MHC class I protein binding; unfolded protein binding; TAP2 binding; peptide antigen-transporting ATPase activity Biological Process: peptide transport; antigen processing and presentation of peptide antigen via MHC class I; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; peptide antigen stabilization; immune response; protein complex assembly; amide transport; antigen processing and presentation of endogenous peptide antigen via MHC class I Disease: Bare Lymphocyte Syndrome, Type I
NCBI Summary:	This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O15533
NCBI GenInfo Identifier:	12643361
NCBI Gene ID:	6892
NCBI Accession:	O15533.1
UniProt Secondary Accession:	O15533,O15210, O15272, Q5STJ8, Q5STK6, Q5STQ5, A2AB91 A2ABC0, B0V003, B0V0A6, B2ZUA4, E9PGM2,
UniProt Related Accession:	O15533
Molecular Weight:	47,626 Da
NCBI Full Name:	Tapasin
NCBI Synonym Full Names:	TAP binding protein (tapasin)
NCBI Official Symbol:	TAPBP
NCBI Official Synonym Symbols:	TPN; TAPA; TPSN; NGS17
NCBI Protein Information:	tapasin; NGS-17; TAP-binding protein; TAP-associated protein
UniProt Protein Name:	Tapasin
UniProt Synonym Protein Names:	NGS-17; TAP-associated protein; TAP-binding protein
UniProt Gene Name:	TAPBP
UniProt Entry Name:	TPSN_HUMAN

	Western blot analysis of extracts of various cell lines, using TAPBP antibody (CAB13357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat liver using TAPBP antibody (CAB13357) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using TAPBP antibody (CAB13357) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using TAPBP antibody (CAB13357). Blue: DAPI for nuclear staining.

Antibodies

Anti-TAPBP Antibody (CAB1968)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)