Anti-SYNE3 Antibody (CAB13237)
- SKU:
- CAB13237
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SYNE3 Antibody |
| 抗体コード: | CAB13237 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 740-920 of human SYNE3 (NP_689805.3). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, |
| ポジティブサンプル: | Jurkat, K-562, LO2, HepG2 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 740-920 of human SYNE3 (NP_689805.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LRLL EESL LSLI RNWH LQRM EVDS GKKM VFTN NIPK SGFL INPM DPIP RHRR RANL LQEE EGSH EDFS QLLR NFGQ WLQV ENSK LVRI IAMR TSTA EDLR TRKS KLQE LEAR VPEG QHLF ENLL RLGP ARGT SDEL EDLR YQWM LYKS KLKD SGHL LTQS SPGE PTGF QKTR RWRG LGSL F |
| 遺伝子ID: | 161176 |
| Uniprot: | Q6ZMZ3 |
| セルラーロケーション: | Nucleus envelope, Nucleus outer membrane, Rough endoplasmic reticulum, Single-pass type IV membrane protein |
| 計算された分子量: | 68kDa/111kDa/112kDa |
| 観察された分子量: | 112kDa |
| 同義語: | SYNE3, C14orf139, C14orf49, KASH3, LINC00341, NCRNA00341, NET53, Nesp3, nesprin-3 |
| バックグラウンド: |
| UniProt Protein Function: | SYNE3: Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow- induced centrosome polarization and directional migration in aortic endothelial cells. Belongs to the nesprin family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 14q32.13 Cellular Component: membrane; nuclear envelope Molecular Function:actin filament binding; protein binding Biological Process: cytoskeleton organization and biogenesis; regulation of cell shape |
| UniProt Code: | Q6ZMZ3 |
| NCBI GenInfo Identifier: | 134035037 |
| NCBI Gene ID: | 161176 |
| NCBI Accession: | Q6ZMZ3.2 |
| UniProt Secondary Accession: | Q6ZMZ3,Q86SX5, Q8N7G8, A6H8H3, |
| UniProt Related Accession: | Q6ZMZ3 |
| Molecular Weight: | 68,582 Da |
| NCBI Full Name: | Nesprin-3 |
| NCBI Synonym Full Names: | spectrin repeat containing nuclear envelope family member 3 |
| NCBI Official Symbol: | SYNE3 |
| NCBI Official Synonym Symbols: | NET53; Nesp3; C14orf49 |
| NCBI Protein Information: | nesprin-3 |
| UniProt Protein Name: | Nesprin-3 |
| UniProt Synonym Protein Names: | Nuclear envelope spectrin repeat protein 3 |
| Protein Family: | Nesprin |
| UniProt Gene Name: | SYNE3 |
| UniProt Entry Name: | SYNE3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SYNE3 antibody (CAB13237) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using SYNE3 Rabbit pAb (CAB13237) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using SYNE3 Rabbit pAb (CAB13237) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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