Anti-Symmetric DiMethyl-Histone H3-R8 Antibody (CAB2374)
(No reviews yet)
Write a Review
- SKU:
- CAB2374
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Application:
- IP
- Application:
- ChIP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Other (Wide Range)
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-Symmetric DiMethyl-Histone H3-R8 Antibody |
| 抗体コード: | CAB2374 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF IP ChIP ChIPseq |
| 反応性: | Human, Mouse, Rat, Other (Wide Range) |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3 |
| 申し込み: | WB IHC IF IP ChIP ChIPseq |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:50 ChIPseq 1:20 - 1:50 |
| 反応性: | Human, Mouse, Rat, Other (Wide Range) |
| ポジティブサンプル: | NIH/3T3 |
| 免疫原: | A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 8290 |
| Uniprot: | Q16695 |
| セルラーロケーション: | Chromosome, Nucleus |
| 計算された分子量: | 15kDa |
| 観察された分子量: | 17kDa |
| 同義語: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A |
| バックグラウンド: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| UniProt Protein Function: | HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q42 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16695 |
| NCBI GenInfo Identifier: | 18202512 |
| NCBI Gene ID: | 8290 |
| NCBI Accession: | Q16695.3 |
| UniProt Secondary Accession: | Q16695,Q6FGU4, B2R5K3, |
| UniProt Related Accession: | Q16695 |
| Molecular Weight: | |
| NCBI Full Name: | Histone H3.1t |
| NCBI Synonym Full Names: | histone cluster 3, H3 |
| NCBI Official Symbol: | HIST3H3 |
| NCBI Official Synonym Symbols: | H3t; H3.4; H3/g; H3FT |
| NCBI Protein Information: | histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T |
| UniProt Protein Name: | Histone H3.1t |
| UniProt Synonym Protein Names: | H3/g |
| UniProt Gene Name: | HIST3H3 |
| UniProt Entry Name: | H31T_HUMAN |
 | Western blot analysis of extracts of NIH/3T3 cells, using Symmetric DiMethyl-Histone H3-R8 antibody (CAB2374) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R8 antibody (CAB2374). |
 | Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R8 antibody (CAB2374). Blue: DAPI for nuclear staining. |
View AllClose