Anti-SUV39H2 Antibody (CAB5855)
- SKU:
- CAB5855
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Application:
- IP
- Application:
- ChIP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SUV39H2 Antibody |
| 抗体コード: | CAB5855 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF IP ChIP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 141-350 of human SUV39H2 (NP_001180354.1). |
| 申し込み: | WB IF IP ChIP |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 ChIP 1:20 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SW620, MCF-7, U-937, 293T, HeLa, Mouse heart, Mouse liver, Mouse eye |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 141-350 of human SUV39H2 (NP_001180354.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | CCPA EAGV LLAY NKNQ QIKI PPGT PIYE CNSR CQCG PDCP NRIV QKGT QYSL CIFR TSNG RGWG VKTL VKIK RMSF VMEY VGEV ITSE EAER RGQF YDNK GITY LFDL DYES DEFT VDAA RYGN VSHF VNHS CDPN LQVF NVFI DNLD TRLP RIAL FSTR TINA GEEL TFDY QMKG SGDI SSDS IDHS PAKK RVRT VCKC GAVT CRGY LN |
| 遺伝子ID: | 79723 |
| Uniprot: | Q9H5I1 |
| セルラーロケーション: | Chromosome, Nucleus, centromere |
| 計算された分子量: | 26kDa/39kDa/46kDa |
| 観察された分子量: | 48kDa |
| 同義語: | SUV39H2, KMT1B |
| バックグラウンド: |
| UniProt Protein Function: | SUV39H2: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation Chromosomal Location of Human Ortholog: 10p13 Cellular Component: nucleoplasm; nuclear heterochromatin; chromatin; chromosome, pericentric region Molecular Function:protein binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K9 specific); chromatin binding Biological Process: chromatin remodeling; chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; rhythmic process; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; male meiosis; cell differentiation |
| UniProt Code: | Q9H5I1 |
| NCBI GenInfo Identifier: | 25091325 |
| NCBI Gene ID: | 79723 |
| NCBI Accession: | Q9H5I1.2 |
| UniProt Secondary Accession: | Q9H5I1,Q5JSS4, Q5JSS5, Q6I9Y3, Q8ND06, D3DRT4, |
| UniProt Related Accession: | Q9H5I1 |
| Molecular Weight: | 410 |
| NCBI Full Name: | Histone-lysine N-methyltransferase SUV39H2 |
| NCBI Synonym Full Names: | suppressor of variegation 3-9 homolog 2 (Drosophila) |
| NCBI Official Symbol: | SUV39H2 |
| NCBI Official Synonym Symbols: | KMT1B |
| NCBI Protein Information: | histone-lysine N-methyltransferase SUV39H2; H3-K9-HMTase 2; su(var)3-9 homolog 2; lysine N-methyltransferase 1B; histone H3-K9 methyltransferase 2 |
| UniProt Protein Name: | Histone-lysine N-methyltransferase SUV39H2 |
| UniProt Synonym Protein Names: | Histone H3-K9 methyltransferase 2; H3-K9-HMTase 2; Lysine N-methyltransferase 1B; Suppressor of variegation 3-9 homolog 2; Su(var)3-9 homolog 2 |
| Protein Family: | Histone-lysine N-methyltransferase |
| UniProt Gene Name: | SUV39H2 |
| UniProt Entry Name: | SUV92_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SUV39H2 antibody (CAB5855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Chromatin immunoprecipitation analysis of extracts of 293T cell line, using SUV39H2 antibody (CAB5855) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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