Anti-SURF1 Antibody (CAB12521)
- SKU:
- CAB12521
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SURF1 Antibody |
| 抗体コード: | CAB12521 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-275 of human SURF1 (NP_003163.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SW480, HL-60, BT-474, SKOV3, Mouse liver, Mouse kidney, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-275 of human SURF1 (NP_003163.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QVQR RKWK LNLI AELE SRVL AEPV PLPA DPME LKNL EYRP VKVR GCFD HSKE LYMM PRTM VDPV REAR EGGL ISSS TQSG AYVV TPFH CTDL GVTI LVNR GFVP RKKV NPET RQKG QIEG EVDL IGMV RLTE TRQP FVPE NNPE RNHW HYRD LEAM ARIT GAEP IFID ANFQ STVP GGPI GGQT RVTL RNEH LQYI |
| 遺伝子ID: | 6834 |
| Uniprot: | Q15526 |
| セルラーロケーション: | Mitochondrion inner membrane, Multi-pass membrane protein |
| 計算された分子量: | 31kDa/33kDa |
| 観察された分子量: | 33kDa |
| 同義語: | SURF1, CMT4K |
| バックグラウンド: | This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency. |
| UniProt Protein Function: | SURF1: Probably involved in the biogenesis of the COX complex. Defects in SURF1 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions that is commonly associated with systemic cytochrome c oxidase (COX) deficiency. Belongs to the SURF1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial Chromosomal Location of Human Ortholog: 9q34.2 Cellular Component: integral to membrane; mitochondrial respiratory chain Molecular Function:protein binding; cytochrome-c oxidase activity Biological Process: ATP biosynthetic process; respiratory chain complex IV assembly; aerobic respiration; oxidative phosphorylation Disease: Leigh Syndrome |
| NCBI Summary: | This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15526 |
| NCBI GenInfo Identifier: | 2498973 |
| NCBI Gene ID: | 6834 |
| NCBI Accession: | Q15526.1 |
| UniProt Secondary Accession: | Q15526,Q5T8T3, Q5T8T4, |
| UniProt Related Accession: | Q15526 |
| Molecular Weight: | 300 |
| NCBI Full Name: | Surfeit locus protein 1 |
| NCBI Synonym Full Names: | surfeit 1 |
| NCBI Official Symbol: | SURF1 |
| NCBI Protein Information: | surfeit locus protein 1 |
| UniProt Protein Name: | Surfeit locus protein 1 |
| Protein Family: | SURF1-like protein |
| UniProt Gene Name: | SURF1 |
| UniProt Entry Name: | SURF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SURF1 antibody (CAB12521) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using SURF1 antibody (CAB12521) at dilution of 1:100 (40x lens). |
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