Anti-SUB1 Antibody (CAB7070)
- SKU:
- CAB7070
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-SUB1 Antibody |
| 抗体コード: | CAB7070 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF IP |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human SUB1 (NP_006704.3). |
| 申し込み: | WB IHC IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HL-60, BT-474, SW620, Jurkat, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human SUB1 (NP_006704.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPKS KELV SSSS SGSD SDSE VDKK LKRK KQVA PEKP VKKQ KTGE TSRA LSSS KQSS SSRD DNMF QIGK MRYV SVRD FKGK VLID IREY WMDP EGEM KPGR KGIS LNPE QWSQ LKEQ ISDI DDAV RKL |
| 遺伝子ID: | 10923 |
| Uniprot: | P53999 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 14kDa |
| 観察された分子量: | 17kDa |
| 同義語: | SUB1, P15, PC4, p14, SUB1 homolog |
| バックグラウンド: |
| UniProt Protein Function: | PC4: a transcriptional coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, nonspecifically to double-stranded DNA (ds DNA). Interacts with CSTF2. Its activity is controlled by phosphorylation of the regulatory domain. Phosphorylation inactivates both dsDNA-binding and cofactor function. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; DNA-binding; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 5p13.3 Cellular Component: transcription factor complex; nucleolus; nucleus Molecular Function:protein binding; transcription coactivator activity; single-stranded DNA binding Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | P53999 |
| NCBI GenInfo Identifier: | 1709514 |
| NCBI Gene ID: | 10923 |
| NCBI Accession: | P53999.3 |
| UniProt Secondary Accession: | P53999,Q96L29, |
| UniProt Related Accession: | P53999 |
| Molecular Weight: | 127 |
| NCBI Full Name: | Activated RNA polymerase II transcriptional coactivator p15 |
| NCBI Synonym Full Names: | SUB1 homolog (S. cerevisiae) |
| NCBI Official Symbol: | SUB1 |
| NCBI Official Synonym Symbols: | P15; PC4; p14 |
| NCBI Protein Information: | activated RNA polymerase II transcriptional coactivator p15; positive cofactor 4; activated RNA polymerase II transcription cofactor 4 |
| UniProt Protein Name: | Activated RNA polymerase II transcriptional coactivator p15 |
| UniProt Synonym Protein Names: | Positive cofactor 4; PC4; SUB1 homolog; p14 |
| Protein Family: | Subtilisin |
| UniProt Gene Name: | SUB1 |
| UniProt Entry Name: | TCP4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SUB1 antibody (CAB7070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human stomach using SUB1 antibody (CAB7070) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using SUB1 antibody (CAB7070). |
 | Immunoprecipitation analysis of 150ug extracts of HL60 cells using 3ug SUB1 antibody (CAB7070). Western blot was performed from the immunoprecipitate using SUB1 antibody (CAB7070) at a dilition of 1:500. |
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