Anti-SS18L1 Antibody (CAB8822)
- SKU:
- CAB8822
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-SS18L1 Antibody |
| 抗体コード: | CAB8822 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-270 of human SS18L1 (NP_945173.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, HT-29, Raji, Mouse brain, Mouse liver, Rat brain, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-270 of human SS18L1 (NP_945173.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LTQS GSSQ GLHS QGSL SDAI STGL PPSS LLQG QIGN GPSH VSMQ QTAP NTLP TTSM SISG PGYS HAGP ASQG VPMQ GQGT IGNY VSRT NINM QSNP VSMM QQQA ATSH YSSA QGGS QHYQ GQSS IAMM GQGS QGSS MMGQ RPMA PYRP SQQG SSQQ YLGQ EEYY GEQY SHSQ GAAE PMGQ Q |
| 遺伝子ID: | 26039 |
| Uniprot: | O75177 |
| セルラーロケーション: | Chromosome, Nucleus, centromere, kinetochore |
| 計算された分子量: | 29kDa/33kDa/40kDa/42kDa/43kDa |
| 観察された分子量: | 55kDa |
| 同義語: | SS18L1, CREST, LP2261 |
| バックグラウンド: | This gene encodes a calcium-responsive transactivator which is an essential subunit of a neuron-specific chromatin-remodeling complex. The structure of this gene is similar to that of the SS18 gene. Mutations in this gene are involved in amyotrophic lateral sclerosis (ALS). Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | SS18L1: Transcriptional activator which is required for calcium- dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the SS18 family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 20q13.3 Cellular Component: kinetochore; nucleus Molecular Function:protein binding Biological Process: positive regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a calcium-responsive transactivator which is an essential subunit of a neuron-specific chromatin-remodeling complex. The structure of this gene is similar to that of the SS18 gene. Mutations in this gene are involved in amyotrophic lateral sclerosis (ALS). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014] |
| UniProt Code: | O75177 |
| NCBI GenInfo Identifier: | 20139981 |
| NCBI Gene ID: | 26039 |
| NCBI Accession: | O75177.2 |
| UniProt Secondary Accession: | O75177,Q5JXJ3, Q6MZV9, Q6NTH3, Q6XYD9, Q8NE69, Q9BR55 Q9H4K6, A6NNE3, A8K620, B3KWR8, E1P5H7, |
| UniProt Related Accession: | O75177 |
| Molecular Weight: | 43,408 Da |
| NCBI Full Name: | Calcium-responsive transactivator |
| NCBI Synonym Full Names: | SS18L1, nBAF chromatin remodeling complex subunit |
| NCBI Official Symbol: | SS18L1 |
| NCBI Official Synonym Symbols: | CREST; LP2261 |
| NCBI Protein Information: | calcium-responsive transactivator |
| UniProt Protein Name: | Calcium-responsive transactivator |
| UniProt Synonym Protein Names: | SS18-like protein 1; SYT homolog 1 |
| Protein Family: | Calcium-responsive transactivator |
| UniProt Gene Name: | SS18L1 |
| UniProt Entry Name: | CREST_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SS18L1 antibody (CAB8822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using SS18L1 antibody (CAB8822) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using SS18L1 antibody (CAB8822) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using SS18L1 antibody (CAB8822) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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