Anti-SPTB Antibody (CAB13048)
- SKU:
- CAB13048
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SPTB Antibody |
| 抗体コード: | CAB13048 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 2080-2180 of human SPTB (NP_001020029.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | K-562, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 2080-2180 of human SPTB (NP_001020029.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QIAE RPAE ETGP QEEE GETA GEAP VSHH AATE RTSP GEEE GTWP QNLQ QPPP PGQH KDGQ KSTG DERP TTEP LFKV LDTP LSEG DEPA TLPA PRDH GQSV Q |
| 遺伝子ID: | 6710 |
| Uniprot: | P11277 |
| セルラーロケーション: | Cytoplasm, cell cortex, cytoskeleton |
| 計算された分子量: | 242kDa/246kDa/267kDa |
| 観察された分子量: | 256kDa |
| 同義語: | SPTB, EL3, HS2, HSPTB1, SPH2, spectrin beta |
| バックグラウンド: | This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | SPTB1: Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Defects in SPTB are the cause of elliptocytosis type 3 (EL3). EL3 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SPTB are the cause of spherocytosis type 2 (SPH2); also known as hereditary spherocytosis type 2 (HS2). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH2 is characterized by severe hemolytic anemia. Inheritance is autosomal dominant. Belongs to the spectrin family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 14q23-q24.2 Cellular Component: spectrin; cell surface; protein complex; cytoplasm; cytosol; actin cytoskeleton Molecular Function:actin filament binding; protein binding; structural constituent of cytoskeleton; protein heterodimerization activity; ankyrin binding; actin binding Biological Process: axon guidance; plasma membrane organization and biogenesis; actin filament capping; hemopoiesis; porphyrin biosynthetic process Disease: Spherocytosis, Type 2, Included |
| NCBI Summary: | This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
| UniProt Code: | P11277 |
| NCBI GenInfo Identifier: | 67782319 |
| NCBI Gene ID: | 6710 |
| NCBI Accession: | NP_000338.3 |
| UniProt Secondary Accession: | P11277,Q15510, Q15519, |
| UniProt Related Accession: | P11277 |
| Molecular Weight: | 246,468 Da |
| NCBI Full Name: | spectrin beta chain, erythrocytic isoform b |
| NCBI Synonym Full Names: | spectrin, beta, erythrocytic |
| NCBI Official Symbol: | SPTB |
| NCBI Official Synonym Symbols: | EL3; HS2; SPH2; HSPTB1 |
| NCBI Protein Information: | spectrin beta chain, erythrocytic; beta-I spectrin; membrane cytoskeletal protein; spectrin beta chain, erythrocyte |
| UniProt Protein Name: | Spectrin beta chain, erythrocytic |
| UniProt Synonym Protein Names: | Beta-I spectrin |
| Protein Family: | Spectrin |
| UniProt Gene Name: | SPTB |
| UniProt Entry Name: | SPTB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPTB antibody (CAB13048) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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