Anti-SPRY4 Antibody (CAB6263)
- SKU:
- CAB6263
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SPRY4 Antibody |
| 抗体コード: | CAB6263 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-210 of human SPRY4 (NP_112226.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa, U-87MG, SW480, HepG2, Mouse heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-210 of human SPRY4 (NP_112226.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | QSAP LTPN SVMV QPLL DSRM SHSR LQHP LTIL PIDQ VKTS HVEN DYID NPSL ALTT GPKR TRGG APEL APTP ARCD QDVT HHWI SFSG RPSS VSSS SSTS SDQR LLDH MAPP PVAD QASP RAVR IQPK VVHC QPLD LKGP AVPP ELDK HFLL CEAC GKCK CKEC ASPR TLPS CWVC NQEC L |
| 遺伝子ID: | 81848 |
| Uniprot: | Q9C004 |
| セルラーロケーション: | Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, ruffle membrane |
| 計算された分子量: | 11kDa/32kDa |
| 観察された分子量: | 37kDa |
| 同義語: | SPRY4, HH17 |
| バックグラウンド: | This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants. |
| UniProt Protein Function: | SPRY4: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Belongs to the sprouty family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Inhibitor; Adaptor/scaffold Chromosomal Location of Human Ortholog: 5q31.3 Cellular Component: cytoplasm; focal adhesion; Golgi apparatus Molecular Function:protein binding Disease: Hypogonadotropic Hypogonadism 17 With Or Without Anosmia |
| NCBI Summary: | This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants. [provided by RefSeq, Jun 2014] |
| UniProt Code: | Q9C004 |
| NCBI GenInfo Identifier: | 14916719 |
| NCBI Gene ID: | 81848 |
| NCBI Accession: | Q9C004.2 |
| UniProt Secondary Accession: | Q9C004,Q6QIX2, Q9C003, A4FVB2, A4FVB3, |
| UniProt Related Accession: | Q9C004 |
| Molecular Weight: | 11,482 Da |
| NCBI Full Name: | Protein sprouty homolog 4 |
| NCBI Synonym Full Names: | sprouty RTK signaling antagonist 4 |
| NCBI Official Symbol: | SPRY4 |
| NCBI Official Synonym Symbols: | HH17 |
| NCBI Protein Information: | protein sprouty homolog 4 |
| UniProt Protein Name: | Protein sprouty homolog 4 |
| Protein Family: | Protein sprouty |
| UniProt Gene Name: | SPRY4 |
| UniProt Entry Name: | SPY4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPRY4 antibody (CAB6263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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