Anti-SPOP Antibody (CAB12106)
- SKU:
- CAB12106
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-SPOP Antibody |
| 抗体コード: | CAB12106 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 175-374 of human SPOP (NP_003554.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:1000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa, HT-29 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 175-374 of human SPOP (NP_003554.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | TMNM VKVP ECRL ADEL GGLW ENSR FTDC CLCV AGQE FQAH KAIL AARS PVFS AMFE HEME ESKK NRVE INDV EPEV FKEM MCFI YTGK APNL DKMA DDLL AAAD KYAL ERLK VMCE DALC SNLS VENA AEIL ILAD LHSA DQLK TQAV DFIN YHAS DVLE TSGW KSMV VSHP HLVA EAYR SLAS AQCP FLGP PRKR LKQS |
| 遺伝子ID: | 8405 |
| Uniprot: | O43791 |
| セルラーロケーション: | Nucleus, Nucleus speckle |
| 計算された分子量: | 42kDa |
| 観察された分子量: | 42kDa |
| 同義語: | SPOP, BTBD32, TEF2 |
| バックグラウンド: | This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. |
| UniProt Protein Function: | SPOP: Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex. In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3. Belongs to the Tdpoz family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 17q21.33 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: proteasomal ubiquitin-dependent protein catabolic process |
| NCBI Summary: | This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43791 |
| NCBI GenInfo Identifier: | 8134708 |
| NCBI Gene ID: | 8405 |
| NCBI Accession: | O43791.1 |
| UniProt Secondary Accession: | O43791,Q53HJ1, B2R6S3, D3DTW7, |
| UniProt Related Accession: | O43791 |
| Molecular Weight: | 42,132 Da |
| NCBI Full Name: | Speckle-type POZ protein |
| NCBI Synonym Full Names: | speckle type BTB/POZ protein |
| NCBI Official Symbol: | SPOP |
| NCBI Official Synonym Symbols: | TEF2; BTBD32 |
| NCBI Protein Information: | speckle-type POZ protein |
| UniProt Protein Name: | Speckle-type POZ protein |
| UniProt Synonym Protein Names: | HIB homolog 1; Roadkill homolog 1 |
| Protein Family: | Speckle-type POZ protein |
| UniProt Gene Name: | SPOP |
| UniProt Entry Name: | SPOP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPOP antibody (CAB12106) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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