Anti-SPHK2 Antibody (CAB6748)
- SKU:
- CAB6748
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SPHK2 Antibody |
| 抗体コード: | CAB6748 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human SPHK2 (NP_001191088.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Raji |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human SPHK2 (NP_001191088.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MNGH LEAE EQQD QRPD QELT GSWG HGPR STLV RAKA MAPP PPPL AAST PLLH GEFG SYPA RGPR FALT LTSQ ALHI QRLR PKPE ARPR GGLV PLAE VSGC CTLR SRSP SDSA AYFC IYTY PRGR RGAR RRAT RTFR ADGA ATYE ENRA EAQR WATA LTCL LRGL P |
| 遺伝子ID: | 56848 |
| Uniprot: | Q9NRA0 |
| セルラーロケーション: | Cytoplasm, Lysosome membrane, Membrane |
| 計算された分子量: | 62kDa/65kDa/69kDa/80kDa |
| 観察された分子量: | 73kDa |
| 同義語: | SPHK2, SK 2, SK-2, SPK 2, SPK-2, SK2, SPK2 |
| バックグラウンド: | This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | SPHK2: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.91 Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: membrane; lysosomal membrane; cytosol Molecular Function:D-erythro-sphingosine kinase activity; protein binding; Ras GTPase binding; sphinganine kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity Biological Process: cell proliferation; blood vessel development; sphingolipid metabolic process; sphingolipid biosynthetic process; protein kinase C activation; positive regulation of cell proliferation; sphingosine metabolic process; sphinganine-1-phosphate biosynthetic process; brain development; lipid phosphorylation; negative regulation of apoptosis |
| NCBI Summary: | This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q9NRA0 |
| NCBI GenInfo Identifier: | 22001996 |
| NCBI Gene ID: | 56848 |
| NCBI Accession: | Q9NRA0.2 |
| UniProt Secondary Accession: | Q9NRA0,Q9BRN1, Q9H0Q2, Q9NWU7, A0T4C8, B4DU87, |
| UniProt Related Accession: | Q9NRA0 |
| Molecular Weight: | 654 |
| NCBI Full Name: | Sphingosine kinase 2 |
| NCBI Synonym Full Names: | sphingosine kinase 2 |
| NCBI Official Symbol: | SPHK2 |
| NCBI Official Synonym Symbols: | SK 2; SK-2; SPK 2; SPK-2 |
| NCBI Protein Information: | sphingosine kinase 2; sphingosine kinase type 2 |
| UniProt Protein Name: | Sphingosine kinase 2 |
| Protein Family: | Sphingosine kinase |
| UniProt Gene Name: | SPHK2 |
| UniProt Entry Name: | SPHK2_HUMAN |
 | Western blot analysis of extracts of Raji cells, using SPHK2 antibody (CAB6748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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