Anti-SPAM1 Antibody (CAB2120)
- SKU:
- CAB2120
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SPAM1 Antibody |
| 抗体コード: | CAB2120 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | PC-3, Raji, BT-474 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | PYID SITG VTVN GGIP QKIS LQDH LDKA KKDI TFYM PVDN LGMA VIDW EEWR PTWA RNWK PKDV YKNR SIEL VQQQ NVQL SLTE ATEK AKQE FEKA GKDF LVET IKLG KLLR PNHL WGYY LFPD CYNH HYKK PGYN GSCF NVEI KRND DLSW LWNE STAL YPSI YLNT QQSP VAAT LYVR NRVR EAIR VSKI PDAK SPLP V |
| 遺伝子ID: | 6677 |
| Uniprot: | P38567 |
| セルラーロケーション: | Cell membrane, GPI-anchor, Lipid-anchor |
| 計算された分子量: | 57kDa/58kDa |
| 観察された分子量: | 47kDa |
| 同義語: | SPAM1, HEL-S-96n, HYA1, HYAL1, HYAL3, HYAL5, PH-20, PH20, SPAG15 |
| バックグラウンド: | Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; EC 3.2.1.35; Membrane protein, GPI anchor; Cell surface Chromosomal Location of Human Ortholog: 7q31.3 Cellular Component: plasma membrane Molecular Function:hyalurononglucosaminidase activity Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; cell adhesion; multicellular organism reproduction |
| NCBI Summary: | Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| UniProt Code: | P38567 |
| NCBI GenInfo Identifier: | 585673 |
| NCBI Gene ID: | 6677 |
| NCBI Accession: | P38567.1 |
| UniProt Secondary Accession: | P38567,Q8TC30, |
| UniProt Related Accession: | P38567 |
| Molecular Weight: | Calculated: 57kDa/58kDaObserved: 47kDa |
| NCBI Full Name: | Hyaluronidase PH-20 |
| NCBI Synonym Full Names: | sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) |
| NCBI Official Symbol: | SPAM1 |
| NCBI Official Synonym Symbols: | HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n |
| NCBI Protein Information: | hyaluronidase PH-20; hyal-PH20; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20; epididymis secretory sperm binding protein Li 96n |
| UniProt Protein Name: | Hyaluronidase PH-20 |
| UniProt Synonym Protein Names: | Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20 |
| Protein Family: | Hyaluronidase |
| UniProt Gene Name: | SPAM1 |
| UniProt Entry Name: | HYALP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPAM1 antibody (CAB2120) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat testis using SPAM1 Rabbit pAb (CAB2120) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using SPAM1 antibody (CAB2120). Blue: DAPI for nuclear staining. |
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