Anti-SPAG8 Antibody (CAB8943)
- SKU:
- CAB8943
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-SPAG8 Antibody |
| 抗体コード: | CAB8943 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SPAG8 (NP_758516.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse testis, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SPAG8 (NP_758516.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LVAD RVPN YTSW SQHC PWEP QKQP PWEF LQVL EPGA RGLW KPPD IKGK LMVC YETL PRGQ CLLY NWEE ERAT NHLD QVPS MQDG SESF FFRH GHRG LLTM QLKS PMPS STTQ KDSY QPPG NVYW PLRG KREA MLEM LLQH QICK EVQA EQEP TRKL FEVE SVTH HDYR MELA QAGT PAPT KPHD YRQE QPET FWIQ RAPQ L |
| 遺伝子ID: | 26206 |
| Uniprot: | Q99932 |
| セルラーロケーション: | Cytoplasmic vesicle, acrosome, secretory vesicle |
| 計算された分子量: | 44kDa/51kDa/53kDa |
| 観察された分子量: | 45kDa |
| 同義語: | SPAG8, BS-84, CILD28, CT142, HSD-1, SMP1, SPAG3, hSMP-1 |
| バックグラウンド: | The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. |
| UniProt Protein Function: | SPAG8: May play a role in fertility and microtubule formation through interaction with RANBP9. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding Biological Process: positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq, Jul 2012] |
| UniProt Code: | Q99932 |
| NCBI GenInfo Identifier: | 62511128 |
| NCBI Gene ID: | 26206 |
| NCBI Accession: | Q99932.2 |
| UniProt Secondary Accession: | Q99932,Q12937, Q5TCV8, Q8WWB4, B4DY89, E9PDV6, |
| UniProt Related Accession: | Q99932 |
| Molecular Weight: | 51,139 Da |
| NCBI Full Name: | Sperm-associated antigen 8 |
| NCBI Synonym Full Names: | sperm associated antigen 8 |
| NCBI Official Symbol: | SPAG8 |
| NCBI Official Synonym Symbols: | SMP1; BS-84; CT142; HSD-1; SPAG3; CILD28; hSMP-1 |
| NCBI Protein Information: | sperm-associated antigen 8 |
| UniProt Protein Name: | Sperm-associated antigen 8 |
| UniProt Synonym Protein Names: | HSD-1; Sperm membrane protein 1; SMP-1; Sperm membrane protein BS-84 |
| Protein Family: | Sperm-associated antigen |
| UniProt Gene Name: | SPAG8 |
| UniProt Entry Name: | SPAG8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPAG8 antibody (CAB8943) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
View AllClose