Anti-SPAG16 Antibody (CAB7482)
- SKU:
- CAB7482
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SPAG16 Antibody |
| 抗体コード: | CAB7482 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human SPAG16 (NP_001020607.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human SPAG16 (NP_001020607.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAQ RGMP SSAV RVLE EALG MGLT AAGD ARDT ADAV AAEG AYYL EQVT ITEA SEDD YEYE EIPD DNFS IPEG EEDL AKAI QMAQ EQAT DTEI LERK TVLP SKHA VPEV IEDF LCNF LIKM GMTR TLDC FQSE WYEL IQKG VTEL RTVG NVPD VYTQ IMLL ENEN KNLK KDLK HYKQ AAEY VIF |
| 遺伝子ID: | 79582 |
| Uniprot: | Q8N0X2 |
| セルラーロケーション: | Cytoplasm, cilium axoneme, cytoskeleton, flagellum axoneme |
| 計算された分子量: | 20kDa/29kDa/39kDa/50kDa/70kDa |
| 観察された分子量: | 71kDa |
| 同義語: | SPAG16, PF20, WDR29 |
| バックグラウンド: |
| UniProt Protein Function: | SPAG16: Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 2q34 Cellular Component: axoneme Biological Process: axoneme biogenesis; cilium biogenesis |
| NCBI Summary: | Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM, Jul 2008] |
| UniProt Code: | Q8N0X2 |
| NCBI GenInfo Identifier: | 146329993 |
| NCBI Gene ID: | 79582 |
| NCBI Accession: | Q8N0X2.2 |
| UniProt Secondary Accession: | Q8N0X2,Q498B7, Q658W1, Q68DB3, Q6I9Z6, Q8N9C7, Q9H601 |
| UniProt Related Accession: | Q8N0X2 |
| Molecular Weight: | 39,739 Da |
| NCBI Full Name: | Sperm-associated antigen 16 protein |
| NCBI Synonym Full Names: | sperm associated antigen 16 |
| NCBI Official Symbol: | SPAG16 |
| NCBI Official Synonym Symbols: | PF20; WDR29 |
| NCBI Protein Information: | sperm-associated antigen 16 protein |
| UniProt Protein Name: | Sperm-associated antigen 16 protein |
| UniProt Synonym Protein Names: | Pf20 protein homolog |
| Protein Family: | Sperm-associated antigen 16 protein |
| UniProt Gene Name: | SPAG16 |
| UniProt Entry Name: | SPG16_HUMAN |
 | Western blot analysis of extracts of mouse testis, using SPAG16 antibody (CAB7482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human brain cancer using SPAG16 antibody (CAB7482) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using SPAG16 antibody (CAB7482) at dilution of 1:100 (40x lens). |
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