Anti-SP100 Antibody (CAB5851)
- SKU:
- CAB5851
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-SP100 Antibody |
| 抗体コード: | CAB5851 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 181-480 of human SP100 (NP_001193631.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, HeLa, 293T, HepG2, Mouse spleen, Mouse thymus, Mouse lung, Mouse heart, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 181-480 of human SP100 (NP_001193631.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | FRSL TWPP SGSP SHAG TTPP ENGL SEHP CETE QINA KRKD TTSD KDDS LGSQ QTNE QCAQ KAEP TESC EQIA VQVN NGDA GREM PCPL PCDE ESPE AELH NHGI QINS CSVR LVDI KKEK PFSN SKVE CQAQ ARTH HNQA SDII VISS EDSE GSTD VDEP LEVF ISAP RSEP VINN DNPL ESND EKEG QEAT CSRP QIVP EPMD FRKL STFR ESFK KRVI GQDH DFSE SSEE EAPA EASS GALR SKHG EKAP MTSR STST WRIP SRKR RFSS SDFS DLSN GEEL QETC SSSL RRGS GKED |
| 遺伝子ID: | 6672 |
| Uniprot: | P23497 |
| セルラーロケーション: | Cytoplasm, Nucleus, PML body |
| 計算された分子量: | 50kDa/53kDa/78kDa/100kDa/101kDa |
| 観察された分子量: | 66-100kDa |
| 同義語: | SP100, lysp100b |
| バックグラウンド: | This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. |
| UniProt Protein Function: | Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to PubMed:11909962. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to PubMed:15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation. |
| NCBI Summary: | This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P23497 |
| NCBI GenInfo Identifier: | 13878931 |
| NCBI Gene ID: | 6672 |
| NCBI Accession: | P23497.3 |
| UniProt Secondary Accession: | P23497,O75450, Q13343, Q8TE34, Q96F70, Q96T24, Q96T95 B4DDX5, B8ZZD8, E7EUA7, E9PH61, F8WFE2, |
| UniProt Related Accession: | P23497 |
| Molecular Weight: | 50,032 Da |
| NCBI Full Name: | Nuclear autoantigen Sp-100 |
| NCBI Synonym Full Names: | SP100 nuclear antigen |
| NCBI Official Symbol: | SP100 |
| NCBI Official Synonym Symbols: | lysp100b |
| NCBI Protein Information: | nuclear autoantigen Sp-100 |
| UniProt Protein Name: | Nuclear autoantigen Sp-100 |
| UniProt Synonym Protein Names: | Nuclear dot-associated Sp100 protein; Speckled 100 kDa |
| Protein Family: | Nuclear autoantigen |
| UniProt Gene Name: | SP100 |
| UniProt Entry Name: | SP100_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SP100 antibody (CAB5851) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
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