Anti-SNF8 Antibody (CAB10583)
- SKU:
- CAB10583
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SNF8 Antibody |
| 抗体コード: | CAB10583 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNF8 (NP_009172.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNF8 (NP_009172.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MHRR GVGA GAIA KKKL AEAK YKER GTVL AEDQ LAQM SKQL DMFK TNLE EFAS KHKQ EIRK NPEF RVQF QDMC ATIG VDPL ASGK GFWS EMLG VGDF YYEL GVQI IEVC LALK HRNG GLIT LEEL HQQV LKGR GKFA QDVS QDDL IRAI KKLK ALGT GFGI IPVG GTYL IQSV PAEL NMDH TVVL QLAE KNGY VTVS EIKA SLKW ETER ARQV LEHL LKEG LAWL DLQA PGEA HYWL PALF TDLY SQEI TAEE AREA LP |
| 遺伝子ID: | 11267 |
| Uniprot: | Q96H20 |
| セルラーロケーション: | Cytoplasm, Endosome membrane, Late endosome membrane, Nucleus |
| 計算された分子量: | 28kDa |
| 観察された分子量: | 30kDa |
| 同義語: | SNF8, Dot3, EAP30, VPS22 |
| バックグラウンド: | The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | SNF8: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Belongs to the SNF8 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: cytoplasm; cytosol; endosome membrane; late endosome membrane; membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome; transcription factor complex Molecular Function:channel regulator activity; protein binding; protein C-terminus binding; protein homodimerization activity; protein N-terminus binding; transcription factor binding Biological Process: early endosome to late endosome transport; endocytic recycling; endosome transport; macroautophagy; positive regulation of protein catabolic process; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; regulation of MAP kinase activity; regulation of protein catabolic process; regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] |
| UniProt Code: | Q96H20 |
| NCBI GenInfo Identifier: | 73919323 |
| NCBI Gene ID: | 11267 |
| NCBI Accession: | Q96H20.1 |
| UniProt Secondary Accession: | Q96H20,Q8IXY3, Q9UN50, |
| UniProt Related Accession: | Q96H20 |
| Molecular Weight: | 29kDa |
| NCBI Full Name: | Vacuolar-sorting protein SNF8 |
| NCBI Synonym Full Names: | SNF8, ESCRT-II complex subunit |
| NCBI Official Symbol: | SNF8 |
| NCBI Official Synonym Symbols: | Dot3; EAP30; VPS22 |
| NCBI Protein Information: | vacuolar-sorting protein SNF8 |
| UniProt Protein Name: | Vacuolar-sorting protein SNF8 |
| UniProt Synonym Protein Names: | ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22 |
| Protein Family: | Vacuolar-sorting protein |
| UniProt Gene Name: | SNF8 |
 | Western blot analysis of extracts of various cell lines, using SNF8 antibody (CAB10583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
View AllClose