Anti-Snail Antibody (CAB5243)
- SKU:
- CAB5243
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Snail Antibody |
| 抗体コード: | CAB5243 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Snail (NP_005976.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, 293T, K-562, NIH/3T3, Mouse lung, Mouse heart, Rat lung |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Snail (NP_005976.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPRS FLVR KPSD PNRK PNYS ELQD SNPE FTFQ QPYD QAHL LAAI PPPE ILNP TASL PMLI WDSV LAPQ AQPI AWAS LRLQ ESPR VAEL TSLS DEDS GKGS |
| 遺伝子ID: | 6615 |
| Uniprot: | O95863 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 29kDa |
| 観察された分子量: | 29kDa |
| 同義語: | SNAI1, SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1 |
| バックグラウンド: | The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. |
| UniProt Protein Function: | Snail1: a protein of the C2H2-type zinc-finger family that regulates transcription. Involved in embryonic mesoderm formation. Plays a role in the epithelial to mesenchymal transition (EMT). EMT is characterized by decreased levels of the epithelial markers E-cadherin and alpha- and beta-catenin, and increased cellular migration and expression of the mesenchymal markers fibronectin, vimentin, N-cadherin and smooth muscle alpha-actin.E Snail binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. NF-kappaB binds and regulates the snail promoter and is critical for EMT, and inhibition of NF-kappaB activity lowered snail levels and morphologically reversed the EMT. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; Transcription factor Chromosomal Location of Human Ortholog: 20q13.2 Cellular Component: cytoplasm; nucleus Molecular Function:kinase binding; protein binding Biological Process: epithelial to mesenchymal transition; mesoderm formation; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; positive regulation of cell migration; positive regulation of transcription, DNA-dependent |
| NCBI Summary: | The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95863 |
| NCBI GenInfo Identifier: | 12644089 |
| NCBI Gene ID: | 6615 |
| NCBI Accession: | O95863.2 |
| UniProt Secondary Accession: | O95863,Q9P113, Q9UBP7, Q9UHH7, B2R842, |
| UniProt Related Accession: | O95863 |
| Molecular Weight: | 29,083 Da |
| NCBI Full Name: | Zinc finger protein SNAI1 |
| NCBI Synonym Full Names: | snail family transcriptional repressor 1 |
| NCBI Official Symbol: | SNAI1 |
| NCBI Official Synonym Symbols: | SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1 |
| NCBI Protein Information: | zinc finger protein SNAI1 |
| UniProt Protein Name: | Zinc finger protein SNAI1 |
| UniProt Synonym Protein Names: | Protein snail homolog 1; Protein sna |
| Protein Family: | Protein snail |
| UniProt Gene Name: | SNAI1 |
| UniProt Entry Name: | SNAI1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Snail antibody (CAB5243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using Snail Rabbit pAb (CAB5243) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of C6 cells using Snail antibody (CAB5243) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using Snail antibody (CAB5243) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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