Anti-SMEK1 Antibody (CAB8500)
- SKU:
- CAB8500
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SMEK1 Antibody |
| 抗体コード: | CAB8500 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 395-594 of human SMEK1 (NP_001271210.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, HepG2, Jurkat, MCF-7, Mouse spleen, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 395-594 of human SMEK1 (NP_001271210.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VQTF KGLK LRFE QQRE RQDN PKLD SMRS ILRN HRYR RDAR TLED EEEM WFNT DEDD MEDG EAVV SPSD KTKN DDDI MDPI SKFM ERKK LKES EEKE VLLK TNLS GRQS PSFK LSLS SGTK TNLT SQSS TTNL PGSP GSPG SPGS PGSP GSVP KNTS QTAA ITTK GGLV GLVD YPDD DEDD DEDE DKED TLPL SKKA KFDS |
| 遺伝子ID: | 55671 |
| Uniprot: | Q6IN85 |
| セルラーロケーション: | Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 62kDa/67kDa/93kDa/95kDa |
| 観察された分子量: | 110kDa |
| 同義語: | PPP4R3A, FLFL1, KIAA2010, MSTP033, PP4R3, PP4R3A, SMEK1, smk-1, smk1 |
| バックグラウンド: |
| UniProt Protein Function: | SMEK1: a conserved single-pass membrane protein. In Dictyostelium, the SMEK homolog regulates myosin II assembly and cytokinesis during vegetative growth. Loss of SMEK suppresses defects in cell polarity, chemotaxis, and gene expression in MEK1 null cells. Six alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q32.12 Cellular Component: cytoplasm; microtubule organizing center; nucleus Biological Process: positive regulation of gluconeogenesis; protein amino acid dephosphorylation |
| UniProt Code: | Q6IN85 |
| NCBI GenInfo Identifier: | 546231920 |
| NCBI Gene ID: | 55671 |
| NCBI Accession: | NP_001271209.1 |
| UniProt Secondary Accession: | Q6IN85,Q69YK6, Q86U23, Q86YI7, Q8IVG1, Q9H3F1, Q9H7U8 Q9NV01, Q9NWP1, |
| UniProt Related Accession: | Q6IN85 |
| Molecular Weight: | 95kDa |
| NCBI Full Name: | serine/threonine-protein phosphatase 4 regulatory subunit 3A isoform 1 |
| NCBI Synonym Full Names: | protein phosphatase 4, regulatory subunit 3A |
| NCBI Official Symbol: | PPP4R3A |
| NCBI Official Synonym Symbols: | smk1; FLFL1; PP4R3; SMEK1; smk-1; PP4R3A; MSTP033; KIAA2010 |
| NCBI Protein Information: | serine/threonine-protein phosphatase 4 regulatory subunit 3A |
| UniProt Protein Name: | Serine/threonine-protein phosphatase 4 regulatory subunit 3A |
| UniProt Synonym Protein Names: | SMEK homolog 1 |
| UniProt Gene Name: | SMEK1 |
| UniProt Entry Name: | P4R3A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SMEK1 antibody (CAB8500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using SMEK1 Rabbit pAb (CAB8500) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using SMEK1 Rabbit pAb (CAB8500) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using SMEK1 Rabbit pAb (CAB8500) at dilution of 1:100 (40x lens). |
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