Anti-SMARCA5 Antibody (CAB2000)
- SKU:
- CAB2000
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SMARCA5 Antibody |
| 抗体コード: | CAB2000 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human SMARCA5 (NP_003592.3). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | PC-3, H460, 293T, HepG2, SH-SY5Y, Mouse spleen, Mouse brain, Mouse testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human SMARCA5 (NP_003592.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ELFA HFIQ PAAQ KTPT SPLK MKPG RPRI KKDE KQNL LSVG DYRH RRTE QEED EELL TESS KATN VCTR FEDS PSYV KWGK LRDY QVRG LNWL ISLY ENGI NGIL ADEM GLGK TLQT ISLL GYMK HYRN IPGP HMVL VPKS TLHN WMSE FKRW VPTL RSVC LIGD KEQR AAFV RDVL LPGE WDVC VTSY EMLI KEKS VFKK FNWR YLVI DEAH RIKN EKSK LSEI VREF KTTN RLLL TGTP LQNN LHEL WSLL NFLL PDVF NSAD DFDS WFDT NNCL GDQK LVER LHMV LRPF LLRR IKAD V |
| 遺伝子ID: | 8467 |
| Uniprot: | O60264 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 121kDa |
| 観察された分子量: | 125kDa |
| 同義語: | SMARCA5, ISWI, SNF2H, WCRF135, hISWI, hSNF2H |
| バックグラウンド: | The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. |
| UniProt Protein Function: | SMARCA5: an ubiquitous enzyme with ATP-dependent DNA helicase activity. Possesses intrinsic ATP-dependent nucleosome remodelling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin in vitro; this may require intact histone H4 tails. The catalytic subunit of the four known chromatin remodelling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy. Belongs to the SNF2/RAD54 helicase family, ISWI subfamily. |
| UniProt Protein Details: | Protein type:Helicase; EC 3.6.4.-; Transcription factor; EC 3.6.1.-; Histone-binding Chromosomal Location of Human Ortholog: 4q31.1-q31.2 Cellular Component: nucleoplasm; chromatin silencing complex; RSF complex; nuclear replication fork; nucleolus; condensed chromosome; nucleus Molecular Function:protein binding; DNA binding; histone binding; nucleosome binding; ATPase activity; helicase activity; ATP binding Biological Process: chromatin silencing at rDNA; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; negative regulation of gene expression, epigenetic; double-strand break repair; transcription initiation; ATP-dependent chromatin remodeling; gene expression; regulation of gene expression, epigenetic; nucleosome positioning |
| NCBI Summary: | The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O60264 |
| NCBI GenInfo Identifier: | 57014128 |
| NCBI Gene ID: | 8467 |
| NCBI Accession: | O60264.1 |
| UniProt Related Accession: | O60264 |
| Molecular Weight: | 1052 |
| NCBI Full Name: | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 |
| NCBI Synonym Full Names: | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 |
| NCBI Official Symbol: | SMARCA5 |
| NCBI Official Synonym Symbols: | ISWI; SNF2H; hISWI; hSNF2H; WCRF135 |
| NCBI Protein Information: | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; sucrose nonfermenting-like 5; sucrose nonfermenting protein 2 homolog; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 |
| UniProt Protein Name: | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 |
| UniProt Synonym Protein Names: | Sucrose nonfermenting protein 2 homolog; hSNF2H |
| Protein Family: | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily |
| UniProt Gene Name: | SMARCA5 |
| UniProt Entry Name: | SMCA5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SMARCA5 antibody (CAB2000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2min. |
 | Immunofluorescence analysis of HeLa cells using SMARCA5 antibody (CAB2000). Blue: DAPI for nuclear staining. |
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