Anti-SLC6A3 Antibody (CAB15236)
- SKU:
- CAB15236
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Neuroscience
Description
| 抗体名: | Anti-SLC6A3 Antibody |
| 抗体コード: | CAB15236 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human SLC6A3 (NP_001035.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, 293T, C6 |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human SLC6A3 (NP_001035.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | GVGQ FSDD IQQM TGQR PSLY WRLC WKLV SPCF LLFV VVVS IVTF RPPH YGAY IFPD WANA LGWV IATS SMAM VPIY AAYK FCSL PGSF REKL AYAI APEK DREL VDRG EVRQ FTLR HWLK V |
| 遺伝子ID: | 6531 |
| Uniprot: | Q01959 |
| セルラーロケーション: | Cell membrane, Multi-pass membrane protein |
| 計算された分子量: | 68kDa |
| 観察された分子量: | 90kDa |
| 同義語: | SLC6A3, DAT, DAT1, PKDYS |
| バックグラウンド: | This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence. |
| UniProt Protein Function: | DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. |
| UniProt Protein Details: | Protein type:Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 5p15.3 Cellular Component: axon; cell soma; cell surface; cytoplasm; flotillin complex; integral to membrane; integral to plasma membrane; plasma membrane Molecular Function:dopamine binding; dopamine transmembrane transporter activity; dopamine:sodium symporter activity; drug binding; monoamine transmembrane transporter activity; protease binding; protein binding; protein complex binding; protein N-terminus binding; protein phosphatase 2A binding; receptor binding Biological Process: adenohypophysis development; aging; dopamine biosynthetic process; dopamine catabolic process; dopamine transport; dopamine uptake; lactation; locomotory behavior; monoamine transport; neurotransmitter biosynthetic process; positive regulation of multicellular organism growth; prepulse inhibition; regulation of dopamine metabolic process; response to cAMP; response to cocaine; response to drug; response to ethanol; response to iron ion; response to nicotine; sensory perception of smell; synaptic transmission; transmembrane transport Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To |
| NCBI Summary: | This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009] |
| UniProt Code: | Q01959 |
| NCBI GenInfo Identifier: | 7108463 |
| NCBI Gene ID: | 6531 |
| NCBI Accession: | AAC50179.2 |
| UniProt Secondary Accession: | Q01959,Q14996, A2RUN4, |
| UniProt Related Accession: | Q01959 |
| Molecular Weight: | 68,495 Da |
| NCBI Full Name: | dopamine transporter |
| NCBI Synonym Full Names: | solute carrier family 6 (neurotransmitter transporter), member 3 |
| NCBI Official Symbol: | SLC6A3 |
| NCBI Official Synonym Symbols: | DAT; DAT1; PKDYS |
| NCBI Protein Information: | sodium-dependent dopamine transporter |
| UniProt Protein Name: | Sodium-dependent dopamine transporter |
| UniProt Synonym Protein Names: | Solute carrier family 6 member 3 |
| Protein Family: | D-alanine aminotransferase |
| UniProt Gene Name: | SLC6A3 |
| UniProt Entry Name: | SC6A3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SLC6A3 antibody (CAB15236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat brain using SLC6A3 antibody (CAB15236) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of rat brain using SLC6A3 Rabbit pAb (CAB15236) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse brain using SLC6A3 Rabbit pAb (CAB15236) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |