Anti-SKAP1 Antibody (CAB3345)
- SKU:
- CAB3345
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-SKAP1 Antibody |
| 抗体コード: | CAB3345 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-358 of human SKAP1 (NP_001068567.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse thymus, Rat thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-358 of human SKAP1 (NP_001068567.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MQAA ALPE EIRW LLED AEEF LAEG LRNE NLSA VARD HRDH ILRG FQQI KARY YWDF QPQG GDIG QDSS DDNH SGTL GLSL TSDA PFLS DYQD EGME DIVK GAQE LDNV IKQG YLEK KSKD HSFF GSEW QKRW CVVS RGLF YYYA NEKS KQPK GTFL IKGY GVRM APHL RRDS KKES CFEL TSQD RRSY EFTA TSPA EARD WVDQ ISFL LKDL SSLT IPYE EDEE EEEK EETY DDID GFDS PSCG SQCR PTIL PGSV GIKE PTEE KEEE DIYE VLPD EEHD LEED ESGT RRKG DYAS YYQG LWDC HGDQ PDEL SFQR GDLI RILS KEYN MYGW WVGE LNSL VGIV PKEY LTTA FEVE ER |
| 遺伝子ID: | 8631 |
| Uniprot: | Q86WV1 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Nucleus |
| 計算された分子量: | 41kDa |
| 観察された分子量: | 55kDa |
| 同義語: | SKAP1, HEL-S-81p, SCAP1, SKAP55 |
| バックグラウンド: | This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. |
| UniProt Protein Function: | SKAP55: Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. Belongs to the SKAP family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: cytoplasm; plasma membrane; intercellular junction; nucleus Molecular Function:protein binding; SH3/SH2 adaptor activity; protein complex binding; SH2 domain binding; SH3 domain binding; protein phosphatase binding; protein kinase binding Biological Process: positive regulation of signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway |
| NCBI Summary: | This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q86WV1 |
| NCBI GenInfo Identifier: | 269849660 |
| NCBI Gene ID: | 8631 |
| NCBI Accession: | Q86WV1.3 |
| UniProt Related Accession: | Q86WV1 |
| Molecular Weight: | |
| NCBI Full Name: | Src kinase-associated phosphoprotein 1 |
| NCBI Synonym Full Names: | src kinase associated phosphoprotein 1 |
| NCBI Official Symbol: | SKAP1 |
| NCBI Official Synonym Symbols: | SCAP1; SKAP55; HEL-S-81p |
| NCBI Protein Information: | src kinase-associated phosphoprotein 1 |
| UniProt Protein Name: | Src kinase-associated phosphoprotein 1 |
| UniProt Synonym Protein Names: | Src family-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa; SKAP-55; pp55 |
| Protein Family: | Src kinase-associated phosphoprotein |
| UniProt Gene Name: | SKAP1 |
| UniProt Entry Name: | SKAP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SKAP1 antibody (CAB3345) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |