Anti-SIRT6 Antibody (CAB7416)
- SKU:
- CAB7416
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SIRT6 Antibody |
| 抗体コード: | CAB7416 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of mouse SIRT6 (NP_001156902.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa, 293T, Mouse spleen, Mouse stomach, A-549, LO2, BT-474, HL-60, Mouse heart |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of mouse SIRT6 (NP_001156902.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | NLQP TKHD RQAD LRIH GYVD EVMC RLMK HLGL EIPA WDGP CVLD KALP PLPR PVAL KAEP PVHL NGAV HVSY KSKP NSPI LHRP PKRV KTEA APS |
| 遺伝子ID: | 50721 |
| Uniprot: | Q8N6T7 |
| セルラーロケーション: | Nucleus, nucleoplasm |
| 計算された分子量: | 36kDa/39kDa |
| 観察された分子量: | 39-45kDa |
| 同義語: | SIRT6, SIR2L6, sirtuin 6 |
| バックグラウンド: | This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Deacetylase; EC 2.4.2.31; EC 3.5.1.-; Transferase Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nuclear telomeric heterochromatin; nucleolus; nucleus Molecular Function:NAD(P)+-protein-arginine ADP-ribosyltransferase activity; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); zinc ion binding; NAD-dependent histone deacetylase activity; chromatin binding; NAD+ ADP-ribosyltransferase activity Biological Process: protein amino acid ADP-ribosylation; histone deacetylation |
| NCBI Summary: | This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q8N6T7 |
| NCBI GenInfo Identifier: | 38258612 |
| NCBI Gene ID: | 51548 |
| NCBI Accession: | Q8N6T7.2 |
| UniProt Related Accession: | Q8N6T7 |
| Molecular Weight: | Observed MW: 39kDa |
| NCBI Full Name: | NAD-dependent protein deacetylase sirtuin-6 |
| NCBI Synonym Full Names: | sirtuin 6 |
| NCBI Official Symbol: | SIRT6 |
| NCBI Official Synonym Symbols: | SIR2L6 |
| NCBI Protein Information: | NAD-dependent protein deacetylase sirtuin-6 |
| UniProt Protein Name: | NAD-dependent protein deacetylase sirtuin-6 |
| UniProt Synonym Protein Names: | Regulatory protein SIR2 homolog 6; SIR2-like protein 6 |
| UniProt Gene Name: | SIRT6 |
| UniProt Entry Name: | SIR6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SIRT6 antibody (CAB7416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of various cell lines, using SIRT6 antibody (CAB7416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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