Anti-SIM2 Antibody (CAB15098)
- SKU:
- CAB15098
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| 抗体名: | Anti-SIM2 Antibody |
| 抗体コード: | CAB15098 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_033664.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| 反応性: | Human |
| ポジティブサンプル: | U-251MG, HepG2 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_033664.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LSLE QVST AKSQ DSWR TALS TSQE TRKL VKPK NTKM KTKL RTNP YPPQ QYSS FQMD KLEC GQLG NWRA SPPA SAAA PPEL QPHS ESSD LLYT PSYS LPFS YHYG HFPL DSHV FSSK KPML PAKF GQPQ GSPC EVAR FFLS TLPA SGEC QWHY ANPL VPSS SSPA KNPP EPPA NTAR HSLV PSYE |
| 遺伝子ID: | 6493 |
| Uniprot: | Q14190 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 63kDa/73kDa |
| 観察された分子量: | 56kDa |
| 同義語: | SIM2, HMC13F06, HMC29C01, SIM, bHLHe15 |
| バックグラウンド: | This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. |
| UniProt Protein Function: | SIM2: Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; DNA-binding Chromosomal Location of Human Ortholog: 21q22.13 Cellular Component: nucleoplasm Molecular Function:signal transducer activity; DNA binding; protein heterodimerization activity; transcription factor activity Biological Process: nervous system development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; cell differentiation; signal transduction; lung development |
| NCBI Summary: | This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
| UniProt Code: | Q14190 |
| NCBI GenInfo Identifier: | 2851630 |
| NCBI Gene ID: | 6493 |
| NCBI Accession: | Q14190.2 |
| UniProt Related Accession: | Q14190 |
| Molecular Weight: | 73kDa |
| NCBI Full Name: | Single-minded homolog 2 |
| NCBI Synonym Full Names: | SIM bHLH transcription factor 2 |
| NCBI Official Symbol: | SIM2 |
| NCBI Official Synonym Symbols: | SIM; bHLHe15; HMC13F06; HMC29C01 |
| NCBI Protein Information: | single-minded homolog 2 |
| UniProt Protein Name: | Single-minded homolog 2 |
| UniProt Synonym Protein Names: | Class E basic helix-loop-helix protein 15; bHLHe15 |
| Protein Family: | Single-minded |
| UniProt Gene Name: | SIM2 |
| UniProt Entry Name: | SIM2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SIM2 antibody (CAB15098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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