Anti-SIGLEC12 Antibody (CAB8519)
- SKU:
- CAB8519
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SIGLEC12 Antibody |
| 抗体コード: | CAB8519 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human SIGLEC12 (NP_443729.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse |
| ポジティブサンプル: | Mouse spleen, Mouse heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human SIGLEC12 (NP_443729.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EQKD YLLT MQKS VTVQ EGLC VSVL CSFS YPQN GWTA SDPV HGYW FRAG DHVS RNIP VATN NPAR AVQE ETRD RFHL LGDP QNKD CTLS IRDT RESD AGTY VFCV ERGN MKWN YKYD QLSV NVTA SQDL LSRY RLEV PESV TVQE GLCV SVPC SVLY PHYN WTAS SPVY GSWF KEGA DIPW DIPV ATNT PSG |
| 遺伝子ID: | 89858 |
| Uniprot: | Q96PQ1 |
| セルラーロケーション: | Membrane, Single-pass type I membrane protein |
| 計算された分子量: | 51kDa/64kDa |
| 観察された分子量: | 80kDa |
| 同義語: | SIGLEC12, S2V, SIGLECL1, SLG, Siglec-XII |
| バックグラウンド: | Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | SIGLEC12: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 19q13.4 |
| NCBI Summary: | Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| UniProt Code: | Q96PQ1 |
| NCBI GenInfo Identifier: | 25009264 |
| NCBI Gene ID: | 89858 |
| NCBI Accession: | Q96PQ1.1 |
| UniProt Secondary Accession: | Q96PQ1,Q8IYH7, |
| UniProt Related Accession: | Q96PQ1 |
| Molecular Weight: | 51,663 Da |
| NCBI Full Name: | Sialic acid-binding Ig-like lectin 12 |
| NCBI Synonym Full Names: | sialic acid binding Ig like lectin 12 (gene/pseudogene) |
| NCBI Official Symbol: | SIGLEC12 |
| NCBI Official Synonym Symbols: | S2V; SLG; SIGLECL1; Siglec-XII |
| NCBI Protein Information: | sialic acid-binding Ig-like lectin 12 |
| UniProt Protein Name: | Sialic acid-binding Ig-like lectin 12 |
| UniProt Synonym Protein Names: | Sialic acid-binding Ig-like lectin-like 1; Siglec-L1 |
| Protein Family: | Sialic acid-binding Ig-like lectin |
| UniProt Gene Name: | SIGLEC12 |
| UniProt Entry Name: | SIG12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SIGLEC12 antibody (CAB8519) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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