Anti-SFN Antibody (CAB18011)[KO Validated]

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SKU:
CAB18011
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
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Description

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抗体名:Anti-SFN Antibody [KO Validated]
抗体コード:CAB18011
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC IF
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SFN (NP_006133.1).
申し込み:WB IHC IF
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:Human, Mouse, Rat
ポジティブサンプル:HeLa
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SFN (NP_006133.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MERA SLIQ KAKL AEQA ERYE DMAA FMKG AVEK GEEL SCEE RNLL SVAY KNVV GGQR AAWR VLSS IEQK SNEE GSEE KGPE VREY REKV ETEL QGVC DTVL GLLD SHLI KEAG DAES RVFY LKMK GDYY RYLA EVAT GDDK KRII DSAR SAYQ EAMD ISKK EMPP TNPI RLGL ALNF SVFH YEIA NSPE EAIS LAKT TFDE AMAD LHTL SEDS YKDS TLIM QLLR DNLT LWTA DNAG EEGG EAPQ EPQS
遺伝子ID:2810
Uniprot:P31947
セルラーロケーション:Cytoplasm, Nucleus, Secreted
計算された分子量:24kDa/27kDa
観察された分子量:27kDa
Anti-SFN Antibody [KO Validated] (CAB18011)Western blot analysis of extracts from normal (control) and SFN knockout (KO) HeLa cells, using SFN antibody (CAB18011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
同義語:SFN, YWHAS, stratifin
バックグラウンド:
UniProt Protein Function:14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; cytosol; nucleus

Molecular Function:protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding

Biological Process: positive regulation of protein export from nucleus; regulation of epidermal cell division; release of cytochrome c from mitochondria; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; negative regulation of caspase activity; regulation of cyclin-dependent protein kinase activity; signal transduction; positive regulation of cell growth

UniProt Code:P31947
NCBI GenInfo Identifier:398953
NCBI Gene ID:2810
NCBI Accession:P31947.1
UniProt Secondary Accession:P31947,Q6FH30, Q6FH51, Q96DH0,
UniProt Related Accession:P31947
Molecular Weight:24,336 Da
NCBI Full Name:14-3-3 protein sigma
NCBI Synonym Full Names:stratifin
NCBI Official Symbol:SFN  
NCBI Official Synonym Symbols:YWHAS  
NCBI Protein Information:14-3-3 protein sigma; 14-3-3 sigma; epithelial cell marker protein 1
UniProt Protein Name:14-3-3 protein sigma
UniProt Synonym Protein Names:Epithelial cell marker protein 1; Stratifin
Protein Family:14-3-3 protein
UniProt Gene Name:SFN  
UniProt Entry Name:1433S_HUMAN
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