Anti-SFN Antibody (CAB1026)
- SKU:
- CAB1026
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SFN Antibody |
| 抗体コード: | CAB1026 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SFN (NP_006133.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, A-549, MCF7, A-431, Mouse lung, Mouse kidney, Rat lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SFN (NP_006133.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MERA SLIQ KAKL AEQA ERYE DMAA FMKG AVEK GEEL SCEE RNLL SVAY KNVV GGQR AAWR VLSS IEQK SNEE GSEE KGPE VREY REKV ETEL QGVC DTVL GLLD SHLI KEAG DAES RVFY LKMK GDYY RYLA EVAT GDDK KRII DSAR SAYQ EAMD ISKK EMPP TNPI RLGL ALNF SVFH YEIA NSPE EAIS LAKT TFDE AMAD LHTL SEDS YKDS TLIM QLLR DNLT LWTA DNAG EEGG EAPQ EPQS |
| 遺伝子ID: | 2810 |
| Uniprot: | P31947 |
| セルラーロケーション: | Cytoplasm, Nucleus, Secreted |
| 計算された分子量: | 24kDa/27kDa |
| 観察された分子量: | 28KDa |
| 同義語: | SFN, YWHAS, stratifin |
| バックグラウンド: |
| UniProt Protein Function: | 14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 1p36.11 Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; cytosol; nucleus Molecular Function:protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding Biological Process: positive regulation of protein export from nucleus; regulation of epidermal cell division; release of cytochrome c from mitochondria; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; negative regulation of caspase activity; regulation of cyclin-dependent protein kinase activity; signal transduction; positive regulation of cell growth |
| UniProt Code: | P31947 |
| NCBI GenInfo Identifier: | 398953 |
| NCBI Gene ID: | 2810 |
| NCBI Accession: | P31947.1 |
| UniProt Secondary Accession: | P31947,Q6FH30, Q6FH51, Q96DH0, |
| UniProt Related Accession: | P31947 |
| Molecular Weight: | 24,336 Da |
| NCBI Full Name: | 14-3-3 protein sigma |
| NCBI Synonym Full Names: | stratifin |
| NCBI Official Symbol: | SFN |
| NCBI Official Synonym Symbols: | YWHAS |
| NCBI Protein Information: | 14-3-3 protein sigma; 14-3-3 sigma; epithelial cell marker protein 1 |
| UniProt Protein Name: | 14-3-3 protein sigma |
| UniProt Synonym Protein Names: | Epithelial cell marker protein 1; Stratifin |
| Protein Family: | 14-3-3 protein |
| UniProt Gene Name: | SFN |
| UniProt Entry Name: | 1433S_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SFN antibody (CAB1026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of various cell lines, using SFN antibody (CAB1026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat skin using SFN Rabbit pAb (CAB1026) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human skin using SFN Rabbit pAb (CAB1026) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse skin using SFN Rabbit pAb (CAB1026) at dilution of 1:100 (40x lens). |
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