Anti-SETDB2 Antibody (CAB7391)
- SKU:
- CAB7391
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-SETDB2 Antibody |
| 抗体コード: | CAB7391 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SETDB2 (NP_001153780.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HL-60, Mouse thymus, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SETDB2 (NP_001153780.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGEK NGDA KTFW MELE DDGK VDFI FEQV QNVL QSLK QKIK DGSA TNKE YIQA MILV NEAT IINS STSI KDPM PVTQ KEQE NKSN AFPS TSCE NSFP EDCT FLTT ENKE ILSL EDKV VDFR EKDS SSNL SYQS HDCS GACL MKMP LNLK GENP LQLP IKCH FQRR HAKT NSHS SALH VSYK TPCG RSLR NVEE VFRY LLET ECNF LFTD NFSF NTYV QLAR NYPK QKEV VSDV DISN GVES VPIS FCNE IDSR KLPQ FKYR KTVW PRAY NLTN FSSM FTDS |
| 遺伝子ID: | 83852 |
| Uniprot: | Q96T68 |
| セルラーロケーション: | Chromosome, Nucleus |
| 計算された分子量: | 80kDa/81kDa |
| 観察された分子量: | 82kDa |
| 同義語: | SETDB2, C13orf4, CLLD8, CLLL8, KMT1F |
| バックグラウンド: | This gene encodes a member of a family of proteins that contain a methyl-CpG-binding domain (MBD) and a SET domain and function as histone methyltransferases. This protein is recruited to heterochromatin and plays a role in the regulation of chromosome segregation. This region is commonly deleted in chronic lymphocytic leukemia. Naturally-occuring readthrough transcription occurs from this gene to the downstream PHF11 (PHD finger protein 11) gene. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | SETDB2: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Methyltransferase, protein lysine; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase Chromosomal Location of Human Ortholog: 13q14 Cellular Component: nucleoplasm; chromosome; nucleus Molecular Function:DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K9 specific) Biological Process: mitosis; establishment and/or maintenance of chromatin architecture; cell division; heart looping; negative regulation of transcription, DNA-dependent; chromosome segregation; histone H3-K9 methylation |
| NCBI Summary: | Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q96T68 |
| NCBI GenInfo Identifier: | 143811459 |
| NCBI Gene ID: | 83852 |
| NCBI Accession: | Q96T68.2 |
| UniProt Secondary Accession: | Q96T68,Q5TC65, Q5TC66, Q5W0A7, Q659A7, Q86UD6, Q96AI6 |
| UniProt Related Accession: | Q96T68 |
| Molecular Weight: | 719 |
| NCBI Full Name: | Histone-lysine N-methyltransferase SETDB2 |
| NCBI Synonym Full Names: | SET domain, bifurcated 2 |
| NCBI Official Symbol: | SETDB2 |
| NCBI Official Synonym Symbols: | CLLD8; CLLL8; KMT1F; C13orf4 |
| NCBI Protein Information: | histone-lysine N-methyltransferase SETDB2; lysine N-methyltransferase 1F; chronic lymphocytic leukemia deletion region 8; chronic lymphocytic leukemia deletion region gene 8 protein |
| UniProt Protein Name: | Histone-lysine N-methyltransferase SETDB2 |
| UniProt Synonym Protein Names: | Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2 |
| Protein Family: | Histone-lysine N-methyltransferase |
| UniProt Gene Name: | SETDB2 |
| UniProt Entry Name: | SETB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SETDB2 antibody (CAB7391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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